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杜 江1, 马振男1, 崔丽艳2, 王德富1, 牛颜冰1*.侵染苘麻的菜豆金色黄花叶病毒的鉴定及基因组结构分析[J].植物保护,2024,50(3):63-69.
侵染苘麻的菜豆金色黄花叶病毒的鉴定及基因组结构分析
Molecular identification and genome sequence analysis of begomoviruses infecting Abutilon theophrasti
投稿时间:2022-11-08  修订日期:2022-12-26
DOI:10.16688/j.zwbh.2022706
中文关键词:  苘麻  菜豆金色黄花叶病毒属  序列分析
英文关键词:Abutilon theophrasti  Begomovirus  sequence analysis
基金项目:山西省基础研究计划(20210302124676);山西农业大学科技创新基金(2020BQ51);山西省高等学校科技创新项目(2021L172);财政部和农业农村部:国家现代农业产业技术体系(CARS-21)
作者单位E-mail
杜 江1, 马振男1, 崔丽艳2, 王德富1, 牛颜冰1* 1. 山西农业大学生命科学学院, 太谷 030801
2. 山西农业大学草业学院, 太谷 030801 
niuyanbingbest@163.com 
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中文摘要:
      为明确引起我国山西晋中地区苘麻叶片表现皱缩和花叶症状的病原物及其基因组分子特征, 本研究利用双生病毒简并引物扩增获得病毒基因组部分序列,经测序、比对后设计特异性引物扩增病毒基因组序列, 进而通过生物信息学方法构建系统发育树并进行序列分析。结果表明:引起苘麻叶片皱缩、花叶的病原物为番茄黄化曲叶病毒(tomato yellow leaf curl virus, TYLCV), 将该分离物命名为TYLCV-Abu, GenBank登录号为OP293347, 但未扩增到β卫星。该病毒DNA-A基因组全长为2 782 bp, 含有6个开放阅读框。TYLCV-Abu分离物与TYLCV茄子分离物KSQ1-3(GenBank登录号KC428753)的核苷酸序列一致性最高, 为98.99%, 其中C4和V2编码的蛋白变异较大。重组结果分析显示,分离物TYLCV-Abu是由TYLCV-F(GenBank登录号KY971326)和TYLCV-KSQ1-3重组得到, 重组区域为其基因组2 617-2 782 nt区域。这是首次从苘麻样品中扩增到TYLCV全基因组序列并进行分析。
英文摘要:
      In order to clarify the pathogen and the genomic sequence characteristics that causing leaf wrinkling and mosaic of Abutilon theophrasti. The samples with typical symptoms were collected from Jinzhong area of Shanxi province, the genome sequence of the virus was amplified using geminivirus degenerate primers and then designed specific primers. The phylogenetic tree was constructed and the virus genomic sequence was analyzed using bioinformatics methods. The results showed that the pathogen causing the leaf wrinkling and mosaic symptoms of A.theophrasti was tomato yellow leaf curl virus (TYLCV), named TYLCV-Abu (GenBank acc. no.OP293347), but no beta satellite amplification was obtained. The viral DNA-A genome was 2 782 bp in length and contained six open reading frames. The TYLCV-Abu sequence shared the highest identity (98.99%) with that of the TYLCV eggplant isolate KSQ1-3 (KC428753), but the proteins encoded by its C4 and V2 genes shared low identities with those of the other virus isolates in GenBank. The results of recombination analysis showed that the TYLCV-Abu isolate was recombined from the TYLCV-F isolate (KY971326) and the TYLCV-KSQ1-3 isolate. The recombination region was from 2 617 nt to 2 782 nt in TYLCV-Abu genome. To our knowledge, this is the first report of the whole genome sequence of TYLCV from A.theophrasti plants.
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