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许常玉, 刘玉娣*, 侯茂林.黑肩绿盲蝽的线粒体基因组结构[J].植物保护,2024,50(3):55-62.
黑肩绿盲蝽的线粒体基因组结构
Structure of the mitochondrial genome of Cyrtorhinus lividipennis Reuter (Hemiptera: Miridae)
投稿时间:2023-05-05  修订日期:2023-07-11
DOI:10.16688/j.zwbh.2023215
中文关键词:  半翅目  盲蝽科  黑肩绿盲蝽  线粒体基因组  系统发育
英文关键词:Hemiptera  Miridae  Cyrtorhinus lividipennis  mitochondrial genome  phylogeny
基金项目:国家重点研发计划(2018YFD0200300)
作者单位E-mail
许常玉, 刘玉娣*, 侯茂林 中国农业科学院植物保护研究所, 植物病虫害综合治理全国重点实验室, 北京 100193 liuyudi@caas.cn 
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中文摘要:
      为研究黑肩绿盲蝽Cyrtorhinus lividipennis的谱系地理和遗传结构, 本文测定并分析了其线粒体基因组全序列。结果表明:黑肩绿盲蝽线粒体基因组全长16 835 bp (GenBank登录号:OK149286), 包括13个蛋白质编码基因(PCGs), 22个tRNA基因, 2个rRNA基因和1个非编码区, 呈闭合双链环状DNA分子结构。线粒体基因组中A+T含量高达76.8%, G+C含量为23.2%, 呈现明显的AT偏向性; 13个蛋白质编码基因均以通用密码子ATN作为起始密码子; 蛋白质编码基因中呈现出明显的氨基酸偏好性, 异亮氨酸含量占比最高, 为10.78%; 22个tRNA基因中有21个tRNA形成典型的三叶草式二级结构; 系统发育树显示, 盲蝽科的11个种聚为两大分支, 黑肩绿盲蝽以99%的自展支持率位于其中的一个分支内。本研究结果将为黑肩绿盲蝽作为天敌昆虫来防治水稻害虫提供帮助。
英文摘要:
      In order to investigate phylogeography and genetic structure of Cyrtorhinus lividipennis, mitochondrial genome of C. lividipennis was sequenced and analyzed. The mitochondrial genome of C. lividipennis is a closed double stranded circular DNA with 16 835 bp in total length (GenBank accession no.OK149286), contains two ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, 13 protein-coding genes and a control region. Nucleotide composition of the mitochondrial genome is biased toward adenine and thymine (A+T) with an AT content of 76.8% and GC content of 23.2%. All 13 protein-coding genes (PCGs) start with a typical ATN initiation codon, showing obvious amino acid preference, and Ile is the most abundant amino acid (10.78%). 21 out of 22 tRNA genes form a typical cloverleaf secondary structure. The phylogenetic tree revealed that the 11 species of the family Miridae clustered into two major branches, and the C. lividipennis clustered in one of the branches with a bootstrap support value of 99%. The results will provide insights into the potential of C. lividipennis as a natural enemy insect for controlling rice pests.
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