| 杨紫薇, 陈宗雄, 丁晓帆*.淡紫紫孢菌PLHN与甲氨基阿维菌素苯甲酸盐减量复配防治象耳豆根结线虫[J].植物保护,2024,50(2):313-324. |
| 淡紫紫孢菌PLHN与甲氨基阿维菌素苯甲酸盐减量复配防治象耳豆根结线虫 |
| Control of Meloidogyne enterolobii by mixture of Purpureocillium lilacinum PLHN and reduction of emamectin benzoate 3% ME |
| 投稿时间：2023-02-15 修订日期：2023-03-18 |
| DOI：10.16688/j.zwbh.2023063 |
| 中文关键词: 象耳豆根结线虫 淡紫紫孢菌 甲维盐 减量 防效 |
| 英文关键词:Meloidogyne enterolobii Purpureocillium lilacinum emamectin benzoate reduction control efficacy |
| 基金项目:2021年度海南省研究生创新科研课题（Qhys2021-55）；2021年度海南大学教改项目（hdjy2163） |
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| 中文摘要: |
| 象耳豆根结线虫Meloidogyne enterolobii严重威胁热带农业生产,迫切需要寻求一种兼具生物防治和化学农药减施高效双重优势的绿色防治方法。本研究通过杀线剂毒力测定和生防菌耐药测定,设计系列药菌复配组合并筛选出对象耳豆根结线虫活性好的复配方案。结果表明:3%甲氨基阿维菌素苯甲酸盐(甲维盐)微乳剂对淡紫紫孢菌Purpureocillium lilacinum PLHN的菌丝生长、产孢量及孢子萌发的影响均较小,且对象耳豆根结线虫的LC50为0.107 mg/L, LC99为0.792 mg/L,绝对致死浓度为0.8 mg/L。复配组合中甲维盐0.4 mg/L(减量50%)+75% PLHN和甲维盐0.6 mg/L(减量25%)+50% PLHN对根结线虫2龄幼虫和卵的抑杀作用均优于其他组合。盆栽防效表明:0.6 mg/L甲维盐(减量25%)+50%PLHN复配组合的防效最好,对根结形成有一定的抑制作用,防治效果与全量甲维盐相当,并能促进番茄植株的生长;0.6 mg/L甲维盐+25% PLHN和0.4 mg/L甲维盐+75%PLHN次之。甲维盐和淡紫紫孢菌PLHN的复配使用,不仅甲维盐能减量25%～50%,还能弥补生物防治稳定性较弱、持效期不长等不足,是植物线虫病防治的可选策略之一。 |
| 英文摘要: |
| Meloidogyne enterolobii is a serious threat to tropical agriculture. It is urgent to find a control method that combines the advantages of biological control, environmental friendliness, and efficient application of chemical pesticides. In this study, the nematocidal activity and resistance of biocontrol strain were measured. A series of compound combinations of nematicide and biocontrol strain were designed, and the optimal combination scheme to control M.enterolobii was selected. The results showed that emamectin benzoate 3% ME had little effect on mycelial growth, sporulation and conidial germination of Purpureocillium lilacinum PLHN. The LC50 and LC99 values of emamectin benzoate against M.enterolobii were only 0.107 mg/L and 0.792 mg/L, respectively, so the absolute lethal concentration was 0.8 mg/L. The inhibitory effects of 0.4 mg/L emamectin benzoate (50% reduction)+75% PLHN and 0.6 mg/L emamectin benzoate (25% reduction)+50% PLHN on the second-stage juveniles (J2) and eggs of the M.enterolobii were superior to those of other combinations. Pot experiment showed that the compound combination of 0.6 mg/L emamectin benzoate+50% PLHN had the best control effect and had a certain inhibitory effect on the formation of root-knot, which was equivalent to the absolute lethal concentration of emamectin benzoate, and could promote the growth of tomato. 0.6 mg/L emamectin benzoate+25% PLHN and 0.4 mg/L emamectin benzoate+75% PLHN took the second place. The combination of emamectin benzoate and P. lilacinum PLHN not only reduced the dose of emamectin benzoate by 25% to 50%, but also made up for the weak stability of biological control and other deficiencies. It is an optional strategy for plant nematode disease control. |
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