| 陈越1, 韩天华3, 马文广3, 钟静1, 尹跃艳2, 赵丽玲1,李婷婷1*, 丁铭1*.云南烟草产区辣椒脉斑驳病毒的调查及其遗传多样性分析[J].植物保护,2024,50(2):192-201. |
| 云南烟草产区辣椒脉斑驳病毒的调查及其遗传多样性分析 |
| Survey and genetic diversity analysis of chilli veinal mottle virus in tobacco-producing areas of Yunnan province |
| 投稿时间：2023-02-28 修订日期：2023-03-31 |
| DOI：10.16688/j.zwbh.2023095 |
| 中文关键词: 辣椒脉斑驳病毒 云南 遗传多样性 遗传结构 cp基因 |
| 英文关键词:chilli veinal mottle virus Yunnan genetic diversity genetic structure cp gene |
| 基金项目:云南省种子种业联合实验室项目(202205AR070001-02)；中国烟草总公司云南省公司科技计划（2021530000242016） |
| 作者 | 单位 | E-mail | | 陈越1, 韩天华3, 马文广3, 钟静1, 尹跃艳2, 赵丽玲1,李婷婷1*, 丁铭1* | 1. 云南省农业科学院生物技术与种质资源研究所, 云南省农业生物技术重点实验室, 农业农村部西南农业基因资源与种质创制重点实验室, 昆明650205
2. 云南省农业科学院高山经济植物研究所, 丽江674100
3. 云南省烟草公司丽江市公司, 丽江674100 | 李婷婷591807832@qq.com；丁铭mingd73@163.com |
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| 中文摘要: |
| 明确辣椒脉斑驳病毒(chilli veinal mottle virus, ChiVMV)在云南6个州(市)烟草生产区的分布、遗传多样性和群体遗传结构,对病害的防控预警具有重要意义。本研究以2022年采集自云南6个州(市)不同烟草产区的96份疑似感染ChiVMV的烟草样品为试验材料,利用电子显微镜负染色观察和分子生物学方法对病毒进行检测和鉴定,获得21个ChiVMV云南烟草分离物的cp基因序列。利用SDT、MEGA、RDP、DnaSP及Arlequin等生物学软件对21个云南烟草分离物及NCBI上下载的38个来自不同国家、地区及寄主的ChiVMV分离物cp基因序列的系统进化、遗传多样性和群体遗传结构特征进行分析。结果显示,从云南6个州(市)烟草生产区采集的96份烟草样品中,ChiVMV检出率为51.04%;序列比对发现,本研究获得的cp基因与NCBI中38个ChiVMV分离物cp基因的核苷酸一致性在84.1%以上;系统发育分析发现,59个ChiVMV分离物按照地理位置的远近被划分为4个分支,聚类结果具有明显的地理分布特征,而与寄主植物无关;遗传多样性和遗传分化分析表明,4个地理种群的ChiVMV cp基因遗传多样性水平均较高,中国种群与印度、泰国和其他国家种群间发生了很大的遗传分化且差异显著(P<0.05);遗传力分析显示,基因交流、遗传漂变和基因的负选择压力是ChiVMV分离物适应性进化的重要方式。 |
| 英文摘要: |
| The objective of this study is to clarify the distribution, genetic diversity, and population genetic structure of chilli veinal mottle virus (ChiVMV) in tobacco production areas across six prefectures (cities) in Yunnan province. It holds great significance for disease prevention, control, and early warning measures. In 2022, 96 tobacco samples suspected of ChiVMV infection were collected from different tobacco production areas in these prefectures (cities). The tobacco samples were detected by electron microscope negative staining observation and molecular biological methods. The cp gene sequences were obtained from 21 ChiVMV Yunnan tobacco isolates. The phylogenetic evolution, genetic diversity and population genetic structure characteristics of the cp gene sequences from the 21 Yunnan tobacco isolates and 38 ChiVMV isolates reported on NCBI, originating from different countries, regions, and hosts, were analyzed using biological software such as SDT, MEGA, RDP, DnaSP, and Arlequin. The results showed that the positive rate of ChiVMV was 51.04% among the 96 tobacco samples collected from the six prefectures (cities) in Yunnan. Sequence alignment revealed nucleotide consistency of the cp gene above 84.1%, with 38 ChiVMV isolates reported on NCBI. Phylogenetic analysis showed that 59 ChiVMV isolates from different sources were categorized into four branches. The clustering results exhibited obvious geographical distribution characteristics unrelated to host plants. Genetic diversity and genetic differentiation analysis revealed high genetic diversity among the four geographical populations of ChiVMV cp gene. Evident genetic differentiation had occurred between the Chinese population and populations from India, Thailand and other countries, with statistically significant differences(P<0.05). Heritability analysis suggested that gene exchange, genetic drift, and negative selection of genes were important adaptive evolution mechanisms for ChiVMV isolates. |
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