| 黄勋1, 丰加文2, 金春林2, 高家卫2, 邓琳梅1,张潇方1, 杨艳丽1*, 刘霞1*.侧孢短芽胞杆菌JYC 688鉴定及抑菌促生特性研究[J].植物保护,2024,50(2):153-162. |
| 侧孢短芽胞杆菌JYC 688鉴定及抑菌促生特性研究 |
| Identification of Brevibacillus laterosporus JYC 688 against potato common scab and its growth promoting characteristics |
| 投稿时间：2022-11-18 修订日期：2023-10-15 |
| DOI：10.16688/j.zwbh.2022730 |
| 中文关键词: 侧孢短芽胞杆菌 鉴定 抑菌特性 促生特性 |
| 英文关键词:Brevibacillus laterosporus identification antimicrobial properties growth promoting characteristics |
| 基金项目:云南省重大科技专项（202102AE090018）;云南省现代农业马铃薯产业技术体系（2021KJTX003）;国家重点研发计划（2018YFD0200808）;云南省农业基础研究联合专项（202301BD070001-063） |
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| 中文摘要: |
| 马铃薯疮痂病是由多种致病链霉菌侵染引起薯块表面结痂导致薯块外观品质下降的病害,防控困难,生物防治是其主要防治措施之一。筛选具有良好拮抗作用的生防菌,可为马铃薯病害的生物防治提供优良的微生物资源。本研究从健康马铃薯植株根际土壤中分离获得JYC 688菌株,采用平板对峙和纸碟法筛选验证菌株拮抗性,结合形态学观察、生理生化特性测定及分子生物学手段确定其种属地位,并检测其产生水解酶和嗜铁载体的能力。结果表明,菌株JYC 688是侧孢短芽胞杆菌Brevibacillus laterosporus,其菌株培养液能够显著抑制引起马铃薯疮痂病的酸性疮痂链霉菌Streptomyces acidiscabies和肿胀疮痂链霉菌S.turgidiscabies的生长,抑菌圈直径分别为27.86 mm和31.90 mm;其对马铃薯晚疫病菌Phytophthora infestans、早疫病菌Alternaria alternata、黑痣病菌Rhizoctonia solani也具有良好的抑制活性;菌株JYC 688能够分泌β-1,3葡聚糖酶、纤维素酶、蛋白酶、嗜铁素等多种抗菌相关物质,可溶解大豆卵磷脂、碳酸钙、碳酸镁3种难溶性化合物,有利于磷、钙、镁3种元素的释放,是一株具有开发潜力的生防菌。 |
| 英文摘要: |
| Potato common scab (PCS) disease is a soil-borne disease caused by a variety of pathogenic Streptomyces spp., which lead to the surface scab and the deterioration of the appearance quality of potato tuber. Screening biocontrol bacteria with good antagonistic effects may provide excellent microbial resources for biological control of potato diseases. In this study,strain JYC 688 was isolated from the rhizosphere soil of healthy potato plants, its antagonistic activity was determined by plate confrontation and paper plate methods, its classification status was identified using morphological observation, physiological and biochemical characterization, and molecular biology methods, its ability to produce hydrolytic enzymes and iron carriers was also tested. The results showed that strain JYC 688 was Brevibacillus laterosporus, and its liquid fermentation could significantly inhibit the growth of Streptomyces acidiscabies and S.turgidiscabies, with inhibition zone diameters of 27.86 mm and 31.90 mm, respectively. It also had good inhibitory activity against Phytophthora infestans, Alternaria alternata and Rhizoctonia solani. The strain JYC 688 could secrete β-1,3 glucanase, cellulase, protease, ferritin, and other antibacterial related substances, and dissolve three insoluble compounds of soybean lecithin, calcium carbonate and magnesium carbonate, which is conducive to the release of three elements of phosphorus, calcium and magnesium. In conclusion, the strain JYC 688 is a potential biocontrol bacterium with good research value. |
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