| 李润红*, 高娜, 孙永军.豆蚜为害对黄芪叶片总叶绿素及营养物质含量的影响[J].植物保护,2024,50(2):93-99. |
| 豆蚜为害对黄芪叶片总叶绿素及营养物质含量的影响 |
| Effects of Aphis craccivora feeding on the contents of total chlorophyll and nutrients in the leaves of Astragalus membranaceus |
| 投稿时间：2023-02-23 修订日期：2023-03-27 |
| DOI：10.16688/j.zwbh.2023080 |
| 中文关键词: 豆蚜 黄芪 为害 叶绿素 营养物质 |
| 英文关键词:Aphis craccivora Astragalus membranaceus infestation chlorophyll nutrient |
| 基金项目:甘肃省教育厅青年博士基金（2021QB-142） |
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| 中文摘要: |
| 为了明确豆蚜与寄主黄芪的互作关系及机理,本研究在人工气候箱内培育黄芪幼苗至15~20 cm左右时,分别接入10日龄成蚜10、20、30头/株和40头/株,并以0头/株作为空白对照,在豆蚜持续取食2、4、6 d和8 d后,分别采集黄芪叶片,测定总叶绿素和营养物质的含量。结果表明,随着虫口密度的增加和取食时间的延长,黄芪叶片内总叶绿素和营养物质含量均发生变化。其中总叶绿素含量随着虫口密度增加和取食时间延长均逐渐下降。在40头/株虫口密度处理下,处理2 d,总叶绿素含量降低至11.06 mg/g,比对照降低41.97%。在相同处理时间下,可溶性蛋白和可溶性糖含量随着虫口密度的增加而降低;在10、20头/株和30头/株虫口密度处理下,可溶性蛋白和可溶性糖含量随取食时间的延长先下降,后升高,再下降,但在40头/株虫口密度处理下,随取食时间延长,可溶性蛋白和可溶性糖含量逐渐下降,处理8 d,其含量为8.39 mg/g和27.33 mg/g,分别比对照下降41.86%和37.79%;在相同处理时间下,游离氨基酸含量随着虫口密度的增加而降低;在10头/株虫口密度处理下,随取食时间的延长先下降,后上升,再下降,其他虫口密度处理下,游离氨基酸含量随取食时间的延长,均呈逐渐递减趋势;在30和40头/株虫口密度处理下,为害2 d,游离氨基酸含量为8.49 μmol/g和7.69 μmol/g,分别比对照降低40.30%和45.92%,以后(4~8 d)其含量与处理2 d时无显著差异(P＞0.05)。综上,随着虫口密度增加和取食时间的延长,黄芪叶片内叶绿素和营养物质含量均发生变化,这对中草药黄芪的蚜虫防治具有参考价值。 |
| 英文摘要: |
| The objective of this study was to clarify the interaction and mechanisms between Aphis craccivora and Astragalus membranaceus. The total chlorophyll and nutrient contents in the leaves of A. membranaceus were determined in an artificial climate chamber, with plants reaching a height of about 15-20 cm. The plants were attacked by 10-day-old adult aphids at densities of 10, 20, 30, 40 aphids/plant, and 0 aphids/plant (CK) for two, four, six, eight days. The results indicated that the total chlorophyl and nutrient contents changed with increasing aphid population density and feeding duration. The total chlorophyll content gradually decreased with an increasing population density of aphids and feeding duration. Specifically, the total chlorophyll content decreased to 11.06 mg/g, which was 41.97% lower than CK at the population density of 40 aphids/plant two days after treatment. The contents of soluble protein and carbohydrates decreased with increasing population density under the same treatment duration. These contents decreased first, followed by an increase, and then decreased again with the extension of aphid feeding time at population densities of 10, 20 aphids/plant, and 30 aphids/plant. However, they gradually decreased at density of 40 aphids/plant. The contents of soluble protein and carbohydrates decreased to 8.39 mg/g and 27.33 mg/g, respectively, at the population density of 40 aphids/plant eight days after treatment, which was 41.86% and 37.79% lower than CK. The content of free amino acids decreased with increasing population density under the same treatment duration. The content decreased first, then increased, and decreased again with the extension of aphid feeding time at density of 10 aphids/plant, but decreased at other population densities. The free amino acids decreased to 8.49 μmol/g and 7.69 μmol/g at the population densities of 30 aphids/plant and 40 aphids/plant two days after treatment, which was 40.30% and 45.92% lower than CK, respectively, but no significant difference was observed between the treatments for 4-8 days and those for two days. In conclusion, the total chlorophyll, soluble protein, soluble carbohydrates, and free amino acid contents changed with an increase in pest population density and the extension of aphid feeding duration. These results provide valuable insights for the control of aphids on A. membranaceus. |
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