| 覃 耀, 杜祖仪, 宋璐丹, 马康生, 万 虎, 李建洪*.褐飞虱对环氧虫啶的抗性选育和遗传方式[J].植物保护,2023,49(5):288-294. |
| 褐飞虱对环氧虫啶的抗性选育和遗传方式 |
| Resistance selection and genetic analysis of Nilaparvata lugens to cycloxaprid |
| 投稿时间：2023-07-10 修订日期：2023-07-28 |
| DOI：10.16688/j.zwbh.2023345 |
| 中文关键词: 褐飞虱 环氧虫啶 抗性选育 抗性遗传 |
| 英文关键词:Nilaparvata lugens cycloxaprid resistance selection resistance inheritance |
| 基金项目:科技基础资源调查专项(2018FY101101) |
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| 中文摘要: |
| 为明确褐飞虱Nilaparvata lugens对环氧虫啶的抗性遗传方式, 进而指导田间合理用药。经过34代选育获得抗环氧虫啶褐飞虱品系(CYC-R), 其抗性水平相较于敏感品系(CYC-S)提高了102.42倍。抗性遗传方式分析结果表明, 环氧虫啶对CYC-R和CYC-S的正、反交后代(F1RS和F1SR)的毒力无显著性差异, 且正、反交后代显性度分别为0.33和0.34, 表明褐飞虱对环氧虫啶的抗性为常染色体、不完全显性遗传。自交和回交的后代(F2RS、F2SR和F2BC)经环氧虫啶处理后的实际死亡率与期望值经卡方检验, 均差异显著, 表明褐飞虱对环氧虫啶的抗性由多基因控制。因此, 一旦褐飞虱田间种群对环氧虫啶产生抗性, 环氧虫啶的使用寿命将会缩短, 抗性治理难度较大, 建议采用预防性抗性治理策略。 |
| 英文摘要: |
| The study aimed to provide insight into the inheritance of resistance in Nilaparvata lugens to cycloxaprid and provide guidance for rational application of pesticides. A cycloxaprid-resistant strain (CYC-R) was obtained through successive selection for 34 generations, with a 102.42-fold increase in resistance compared to the laboratory susceptible strain (CYC-S). There was no significant difference in LC50 values of cycloxaprid to progenies (F1RS and F1SR) of reciprocal crosses between CYC-R and CYC-S strains, and the dominance degrees of F1RS and F1SR were 0.33 and 0.34, respectively, indicating that cycloxaprid-resistance in N.lugens was autosomal and incompletely dominant. Based on Chi-square goodness-of-fit test, significant difference was found between the observed and expected mortalities of F2RS, F2SR and F2BC, indicating that cycloxaprid-resistance in N.lugens is a polygenic effect. Therefore, once N.lugens has developed resistance to cycloxaprid in the field, the service life of cycloxaprid will be shortened and the resistance will be difficult to manage, so it is recommended that a preventive resistance management strategy should be implemented. |
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