| 王雨1, 王鑫伟1, 刘洋1, 刘楚涵2, 钱怡云1, 宋萍萍1*, 韦敏1*.汉防己甲素对5种植物病原真菌的抑制作用及对灰葡萄孢的作用机制初探[J].植物保护,2023,49(3):298-304. |
| 汉防己甲素对5种植物病原真菌的抑制作用及对灰葡萄孢的作用机制初探 |
| Inhibitory effect of tetrandrine on five plant pathogens and its mechanism on Botrytis cinerea |
| 投稿时间：2022-06-18 修订日期：2022-09-04 |
| DOI：DOI: 10.16688/j. zwbh. 2022343 |
| 中文关键词: 汉防己甲素 植物病原真菌 灰葡萄孢 钙离子 钙离子通道 |
| 英文关键词:tetrandrine plant pathogens Botrytis cinerea Ca2+ Ca2+ channel |
| 基金项目:国家自然科学基金青年科学基金（31801793） |
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| 中文摘要: |
| 为寻找环境友好型的农药先导化合物, 利用菌丝生长速率法研究了粉防己中汉防己甲素对常见植物病原真菌的抑制活性, 结果表明, 汉防己甲素对5种常见植物病原真菌均有抑制作用, 对立枯丝核菌的抑制作用最强, EC50为33.49 mg/L; 对串珠镰孢和瓜类黑腐球壳菌的EC50分别为41.71 mg/L和43.97 mg/L, 抑制活性优于对照植物源药剂蛇床子素; 对灰葡萄孢的抑制活性与蛇床子素相当。接种蓝莓离体叶片的试验结果表明, 汉防己甲素对蓝莓灰霉病具有治疗和保护作用且作用效果与对照药剂蛇床子素基本相当; 激光共聚焦显微镜检测发现汉防己甲素可降低灰葡萄孢菌丝内钙离子浓度, 汉防己甲素分别与CaCl2及钙通道阻滞剂维拉帕米两者相互作用可降低灰葡萄孢对各药剂的敏感性, 表明其作用机制与钙离子及钙离子通道有关。本文首次报道汉防己甲素对5种植物病原真菌的抑制作用及其作用机制, 为其进一步的开发应用提供理论基础。 |
| 英文摘要: |
| In order to search for more effective environment friendly fungicide lead compounds, sensitivity of tetrandrine on five plant pathogens were determined by mycelium growth rate method. The results showed that tetrandrine had antifungal activity against all tested pathogens, displayed a strong antifungal activity against Rhizoctonia solani isolate (EC50=33. 49 mg/L), exhibited a higher activity against Fusarium moniliforme isolate (EC50=41.71 mg/L) and Didymella bryoniae isolate (EC50=43. 97 mg/L) than osthole, and showed significant activity against Botrytis cinerea equal to osthole. The results of inoculation of blueberry leaves in vitro showed that tetrandrine had both therapeutic and protective activities against gray mold infections, equal to those of osthole. Laser confocal microscopy showed that tetrandrine could reduce the concentration of calcium ions in the mycelia of B. cinerea, and the interaction of tetrandrine with CaCl2 and calcium channel blocker verapamil could reduce the sensitivity of B. cinerea to each reagent, indicating that the mechanism of tetrandrine against B. cinerea was related to Ca2+ and Ca2+ channels. Our work represents the first report of the antifungal properties of tetrandrine against five plant pathogens and its mechanism, providing a theoretical basis for its further development and application. |
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