| 郑晨曦1, 程鹏2, 丁伟1*.水稻苗床α 萘乙酸药害的生物修复[J].植物保护,2023,49(3):141-149. |
| 水稻苗床α 萘乙酸药害的生物修复 |
| Bioremediation of α naphthylacetic acid damage in rice seedbeds |
| 投稿时间：2022-02-18 修订日期：2022-04-10 |
| DOI：DOI: 10.16688/j. zwbh. 2022084 |
| 中文关键词: 水稻 α 萘乙酸 药害 降解 生物修复 |
| 英文关键词:rice α naphthylacetic acid drug damage degradation bioremediation |
| 基金项目:黑龙江省科技攻关项目（GC12B103） |
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| 中文摘要: |
| α 萘乙酸广泛用于水稻秧田调节根系生长, 常因用量控制不当造成水稻根系畸形, 生长被抑制, 地上部叶片变黄等药害大面积发生。从连续定量施用α 萘乙酸的土壤中分离获得6株菌株, 确定其中2株菌株对α 萘乙酸药害具有明显修复作用, 经细菌16S rDNA和真菌 rDNA-ITS鉴定, 细菌1号为芽胞杆菌Bacillus sp. , 真菌2号为蓝状菌Talaromyces sp. 。细菌1号和真菌2号菌剂施用后21 d, 对苗床土壤中α 萘乙酸的降解率分别为56.15%和38.06%; 细菌1号处理下, 水稻4叶期株高、茎基部宽度、须根数及根系干重分别恢复至对照的84.1%、86.1%、97.7%和84.5%, 叶片SOD和POD活性分别显著提高36.4%和66.8%, MDA含量明显降低20.5%; 真菌2号处理下, 水稻4叶期, 株高、茎基部宽度、须根数及根系干物质分别恢复至对照的77.7%、81.6%、92.5%和80.4%。叶片SOD和POD活性分别提高11.1%和74.4%, MDA含量显著降低10.6%。综上，细菌1号和真菌2号对水稻苗床α 萘乙酸药害具有显著修复效果。 |
| 英文摘要: |
| Alpha naphthalene acetic acid is widely used in rice seedling fields to regulate root growth, often causing root deformities, growth inhibition and above ground leaf yellowing due to improper dosage control. Six microbial strains were isolated from soil under continuous quantitative application of α naphthylacetic acid, and two of them were determined to have significant remediation effect on α naphthylacetic acid damage, identified by 16S rDNA and rDNA-ITS as Bacillus pacificus (Bacteria 1) and Talaromyces amestolkiae (Fungus 2). The degradation rate of α naphthylacetic acid in the soil of the seedbed by Bacteria 1 and Fungus 2 was 56.15% and 38.06% 21 days after applications, respectively. The plant height, stem basal width, fibrous root number and root dry weight of rice at the 4 leaf stage recovered to 84.1%, 86.1%, 97.7% and 84.5% of the control under Bacterial 1 treatment, and leaf SOD and POD activity were significantly increased by 36.4% and 66.8%, and the MDA content was obviously reduced by 20.5%. At the 4 leaf stage of rice under Fungus 2 treatment, plant height, stem basal width, number of fibrous roots and root dry matter recovered to 77.7%, 81.6%, 92.5% and 80.4% of the control, respectively. Leaf SOD and POD activities were increased by 11.1% and 74.4%, respectively, and MDA content was significantly reduced by 10.6%. It suggested that Bacteria 1 and Fungus 2 had significant restoration effect on α naphthalene acetic acid damage in rice seedbeds. |
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