| 余永婷1, 李水根1, 方献平1, 张昊2, 安海山1, 张学英1*, 张丽勍1*.草莓果生炭疽菌LysM效应子CfLysM2的致病功能分析[J].植物保护,2023,49(3):39-49. |
| 草莓果生炭疽菌LysM效应子CfLysM2的致病功能分析 |
| Functional analysis of the LysM effector CfLysM2 in the pathogenicity of Colletotrichum fructicola on strawberry |
| 投稿时间：2022-02-14 修订日期：2022-05-22 |
| DOI：DOI: 10.16688/j. zwbh. 2022074 |
| 中文关键词: 果生炭疽菌 LysM 结构域 致病性 转录组 |
| 英文关键词:Colletotrichum fructicola LysM domain pathogenicity transcriptome |
| 基金项目:上海市科委启明星人才计划（18QB1402800） |
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| 中文摘要: |
| 果生炭疽菌Colletotrichum fructicola侵染引起的草莓炭疽病是草莓生产中的重要病害。前期研究发现一个在果生炭疽菌侵染阶段高表达的含LysM结构域的候选效应子CfLysM2。为进一步研究CfLysM2的致病功能, 运用同源重组方法构建CfLysM2缺失突变株ΔCfLysM2和回补菌株CfLysM2c。致病力测定结果表明, 接种后15 d, ΔCfLysM2接种的草莓叶片病情指数显著下降。荧光定量PCR分析表明CfLysM2基因在果生炭疽菌接种草莓叶片后24 h相对表达量最高。利用转录组技术对野生型菌株(wild type strain, WT)和ΔCfLysM2接种后24 h的草莓叶片样品进行比较分析, 获得差异表达基因109个。CfLysM2的缺失导致宿主代谢途径尤其是次生代谢物生物合成通路的激活, 说明CfLysM2可能在果生炭疽菌侵染草莓过程中通过靶向黄酮醇合成酶等代谢相关基因, 抑制植物次生代谢产物合成及其介导的抗真菌免疫, 保证其顺利侵染。该研究为揭示CfLysM2机理及对果生炭疽菌和草莓互作研究奠定了理论基础。 |
| 英文摘要: |
| Strawberry anthracnose caused by Colletotrichum fructicola is an important disease in strawberry production. A candidate effector CfLysM2 containing LysM domain highly expressed during the infection of Colletotrichum fructicola was identified in the previous study. To further study the pathogenic function of CfLysM2, a CfLysM2 deletion mutant ΔCfLysM2 and a complementary strain CfLysM2c were constructed by homologous recombination. The results of pathogenicity assay showed that the disease index decreased significantly 15 days post inoculation with the mutant strain. Real time quantitative PCR analysis showed that the relative expression of CfLysM2 was the highest 24 h post infection by C. fructicola in strawberry leaves. The transcriptome technology was used to compare and analyze strawberry leaf samples 24 h after inoculation of wild type strain and mutant strain ΔCfLysM2, respectively. The results showed that there were 109 differentially expressed genes. Knockout of CfLysM2 led to the activation of host metabolic pathways, especially the biosynthetic pathway of secondary metabolites, indicating that CfLysM2 might inhibit the synthesis of plant secondary metabolites and metabolite mediated antifungal immunity to ensure its successful infection by targeting metabolism related genes such as flavonol synthase during infection. This study lays a theoretical foundation for revealing the functional mechanism of CfLysM2 and for the study of the interaction between C. fructicola and strawberry. |
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