| 冯柃杰#, 张玲玉#, 王晓东, 翟彤, 甘鹏飞, 汤春蕾, 康振生, 王晓杰*.蛋白激酶TaPiPK1在小麦抗条锈病反应中的功能分析[J].植物保护,2023,49(3):32-38. |
| 蛋白激酶TaPiPK1在小麦抗条锈病反应中的功能分析 |
| Functional analysis of protein kinase TaPiPK1 in wheat resistance response to stripe rust |
| 投稿时间：2022-04-01 修订日期：2022-05-09 |
| DOI：DOI: 10.16688/j. zwbh. 2022172 |
| 中文关键词: 小麦 条锈病 蛋白激酶 病毒诱导的基因沉默 广谱抗性 |
| 英文关键词:wheat stripe rust protein kinase VIGS broad spectrum resistance |
| 基金项目:陕西省创新人才推进计划——科技创新团队（2018TD-004） |
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| 中文摘要: |
| 由条形柄锈菌小麦专化型Puccinia striiformis f. sp. tritici (Pst)引起的条锈病长期严重威胁小麦安全生产。Pst通过毒性变异产生新毒性小种或致病类型, 能克服生产上已应用的抗病基因, 导致抗病品种感病。因此, 挖掘抗条锈病基因资源对小麦抗病遗传改良和加快抗病新品种培育具有重要意义。蛋白激酶在应答病原菌侵染, 传递植物免疫信号中发挥重要作用。本研究通过小麦转录组数据分析, 筛选到参与应答Pst、小麦白粉菌Blumeria graminis f. sp. tritici和赤霉病菌Fusarium graminearum侵染的蛋白激酶基因TaPiPK1。该蛋白激酶基因编码551个氨基酸, C末端含有一个STK激酶结构域。利用RT-qPCR检测发现, TaPiPK1在小麦与条锈菌非亲和互作的前期上调表达; TaPiPK1主要定位于细胞质与细胞膜。利用大麦条纹花叶病毒介导的基因沉默技术(BSMV VIGS)瞬时沉默TaPiPK1，显著降低小麦‘水源11’对条锈菌非亲和小种CYR23的抗性, 表明TaPiPK1参与小麦抗条锈病反应并发挥重要作用。因此, TaPiPK1有可能作为潜在的基因资源用于小麦抗条锈病育种。 |
| 英文摘要: |
| Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a serious threat to wheat production. Pst frequently produces new virulent races or pathogenic types overcoming the disease resistant genes applied in wheat production through frequent virulence variation, subsequently leading to the susceptibility of resistant varieties to diseases. Therefore, excavating genetic resources of resistance is of great significance for the genetic improvement of wheat disease resistance and accelerating disease resistance breeding. Protein kinases play an important role in the transmission of plant immune signals in response to pathogen infection. In this study, a protein kinase gene TaPiPK1 involved in response to Pst, Blumeria graminis f. sp. tritici and Fusarium graminearum was identified by analyzing wheat transcriptome data. TaPiPK1 encoded 551 amino acids, and contained a serine threonine kinase domain at C terminal. RT-qPCR results showed that the expression of TaPiPK1 was upregulated in the early stage of incompatible interaction between wheat and Pst. Subcellular localization revealed that TaPiPK1 was mainly localized in cytoplasm and cell membrane. Transient silencing of TaPiPK1 by barley stripe mosaic virus mediated gene silencing technique (BSMV VIGS) significantly reduced the resistance of wheat ‘Suwon 11’ to Pst CYR23, indicating that TaPiPK1 plays an important role in wheat stripe rust resistance. Therefore, TaPiPK1 may be a potentially important gene resource for breeding for stripe rust resistance in wheat. |
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