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刘海娇1#, 徐 杰1#, 左登鸿1, 苏应威1, 朱书生1, 2, 杨 敏1, 2*.茼蒿水浸液和挥发物对三七根腐病菌的抑制活性及其抑菌物质分析[J].植物保护,2023,49(2):206-214.
茼蒿水浸液和挥发物对三七根腐病菌的抑制活性及其抑菌物质分析
Inhibitory activities and antifungal substances of water extracts and volatiles from Glebionis coronaria on root rot pathogens of Panax notoginseng
投稿时间:2022-01-12  修订日期:2022-03-27
DOI:10.16688/j.zwbh.2022019
中文关键词:  茼蒿  三七  挥发物  水浸液  根腐病
英文关键词:Glebionis coronaria  Panax notoginseng  volatiles  water extracts  root rot
基金项目:云南农业大学林下中药材有机种植省创新团队(202105AE160016); 云南省李健强专家工作站(202105AF150046); 国家自然科学基金(32060719); 云南省中青年后备人才项目(202005AC160045)
作者单位E-mail
刘海娇1#, 徐 杰1#, 左登鸿1, 苏应威1, 朱书生1, 2, 杨 敏1, 2* LIU Haijiao1#, XU Jie1#, ZUO Denghong1, SU Yingwei1, ZHU Shusheng1, 2, YANG Min1, 2* yangminscnc@126.com 
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中文摘要:
      为明确茼蒿水浸液和挥发物对三七根腐病菌的抑制活性及主要的抑菌物质, 采用菌丝生长速率法测定其对腐皮镰刀菌Fusarium solani F-3和锈腐病菌Ilyonectria destructans RS006的抑制活性, 利用气相色谱-质谱联用仪分析水浸液和挥发物的成分, 进一步验证化合物的抑菌活性?结果表明, 茼蒿茎?叶水浸液对F-3的抑制效果强于RS006, 叶水浸液比茎水浸液抑菌效果更好?叶水浸液浓度为15.0%时, 对F-3的抑制率为55.1%?挥发物对RS006的抑制活性存在明显的浓度效应, 茎叶用量为2.0 g/皿时, 茎和叶挥发物对RS006的抑制率均高于85.0%?茼蒿茎挥发物对F-3的抑制率较低且不同浓度间差异不显著, 叶挥发物在叶用量为1.0 g/皿时对F-3抑制效果最好, 抑制率为35.3%?茼蒿茎水浸液的主要成分为糖类和有机酸类, 叶水浸液的主要成分为有机酸类?茼蒿茎叶挥发物的主要成分为萜烯类?挥发物中芳樟醇对两种病原菌的抑制效果较好?利用茼蒿或其化感物质可为三七根腐病的绿色防控提供思路?
英文摘要:
      This study aimed to determine the inhibition activity and the main antifungal substances of water extracts and volatiles from Glebionis coronaria on Panax notoginseng root rot pathogens. The inhibitory activities against Fusarium solani F-3 and Ilyonectria destructans RS006 were determined by mycelium growth rate method, and the substances of the aqueous extracts and volatiles were analyzed by gas chromatography-mass spectrometry, and the antifungal activity of the compounds were further verified. The results showed that the inhibitory effect of water extracts from stems and leaves on F-3 was stronger than that of RS006, and the inhibitory effect of leaf extracts was better than that of stems extracts. When the concentration of leaf water extracts was 15.0%, the inhibition rate on F-3 was 55.1%. The inhibition rate of volatiles on RS006 was significantly concentration-dependent, and the inhibition rate were higher than 85.0% at 2.0 g stems or leaves per dish. The inhibition rates of volatiles from stems on F-3 were low and there was no significant difference among different concentrations. The inhibition rate on F-3 was 35.3% when the leaf was 1.0 g per dish. The main components of stem water extracts of G. coronaria were sugars and organic acids, while the main components of leaf water extracts were organic acids. The volatiles of G. coronaria were mainly composed of terpenes. Linalool in volatiles had obvious inhibitory effect on the two pathogens. G. coronaria or its allelopathic substances could be used for prevention and ecological control of P. notoginseng root rot disease.
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