| 杨 林, 刘彩月, 韩小强*, 陈明慧, 陈阅新, 张艳聪, 周科瑾.虱螨脲对棉铃虫叶酸一碳库代谢途径的影响[J].植物保护,2023,49(2):65-72. |
| 虱螨脲对棉铃虫叶酸一碳库代谢途径的影响 |
| Effects of lufenuron on the folate and one-carbon unit metabolism pathway in Helicoverpa armigera |
| 投稿时间：2021-11-10 修订日期：2022-01-05 |
| DOI：10.16688/j.zwbh.2021626 |
| 中文关键词: 虱螨脲 棉铃虫 叶酸一碳库 转录组 分子对接 |
| 英文关键词:lufenuron Helicoverpa armigera one-carbon pool by folate transcriptome molecular docking |
| 基金项目:石河子大学创新拔尖人才项目(CXBJ202007) |
| 作者 | 单位 | E-mail | | 杨 林, 刘彩月, 韩小强*, 陈明慧, 陈阅新, 张艳聪, 周科瑾 | YANG Lin, LIU Caiyue, HAN Xiaoqiang*, CHEN Minghui, CHEN Yuexin, ZHANG Yancong, ZHOU Kejin | hanshz@shzu.edu.cn |
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| 中文摘要: |
| 为了探究虱螨脲对棉铃虫的作用机理, 用虱螨脲(200 mg/L)处理棉铃虫3龄幼虫, 通过转录组测序?叶酸含量测定?叶酸饲喂试验和分子对接技术分析了虱螨脲对棉铃虫叶酸一碳库代谢途径的影响?结果表明, 棉铃虫幼虫差异基因数量随虱螨脲处理后时间的延长而增多, 主要富集在叶酸一碳库?氧化还原活性?几丁质结合?氨基酸合成?嘌呤合成与代谢以及离子跨膜运输等途径上, 其中叶酸一碳库代谢通路在处理后0.5 h极显著富集(P=6.35×10-5)?棉铃虫幼虫的叶酸含量随着虱螨脲处理后时间的延长而显著降低, 处理后24 h叶酸含量为4.53 μg/100 g, 约为对照组的1/2; 用含叶酸的饲料饲喂虱螨脲处理的棉铃虫幼虫, 能够明显降低其中毒症状?分子对接结果表明, 胞质10-甲酰基四氢叶酸脱氢酶(cytosolic 10-formyltetrahydrofolate dehydrogenase)与虱螨脲共形成12个氢键和6个卤键, 具有最低的结合能(-10.4 kcal/mol), 推测其是虱螨脲的潜在作用靶标之一?研究结果进一步丰富了虱螨脲的作用机理, 为以叶酸途径为靶标的杀虫剂开发提供了理论指导? |
| 英文摘要: |
| In order to explore the action mechanism of lufenuron on Helicoverpa armigera, the 3rd instar larvae of H. armigera were treated with lufenuron (200 mg/L). The effects of lufenuron on folate and one-carbon metabolism of H. armigera were analyzed by transcriptome sequencing, folate content determination, folate feeding test and molecular docking technology. The number of differentially expressed genes (DEGs) increased with the extension of time after treatment, and their functions were mainly enriched in one-carbon pool by folate, oxidation-reduction process, chitin binding, amino acid synthesis, purine synthesis and metabolism, and ion transport across membranes. Among them, the one-carbon pool by folate pathway (P=6.35×10-5) was extremely significantly enriched in 0.5 h after treatment. The folate content in H. armigera larvae decreased significantly after treated with lufenuron, and the folate content of H. armigera treated with lufenuron was 4.53 μg/100 g after 24 h, about 1/2 of the control. The poisoning symptoms of H. armigera treated with lufenuron were obviously reduced by feeding H. armigera with folate. Molecular docking results showed that cytosolic 10-formyltetrahydrofolate dehydrogenase formed 12 hydrogen bonds and 6 halogen bonds with lufenuron. They had the lowest binding energy (-10.4 kcal/mol), indicating that they were the potential targets of lufenuron. Our study helps to understand the action mechanism of lufenuron, and provides a theoretical guidance for the development of insecticides targeting folate pathway. |
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