| 祁山颜1, 2#, 朱 峰2#, 王继春2*, 田成丽2, 王东元1, 欧玉苹1, 2, 刘晓梅2, 李 莉2, 姜兆远2.枯草芽胞杆菌GB519在水稻植株中的定殖及对稻瘟病田间防效[J].植物保护,2023,49(2):48-56. |
| 枯草芽胞杆菌GB519在水稻植株中的定殖及对稻瘟病田间防效 |
| Colonization dynamics of Bacillus subtilis GB519 in rice plants and its biocontrol efficacy against rice blast in the field |
| 投稿时间：2021-12-27 修订日期：2022-02-17 |
| DOI：10.16688/j.zwbh.2021719 |
| 中文关键词: 枯草芽胞杆菌GB519 GFP 定殖动态 稻瘟病 防效 |
| 英文关键词:Bacillus subtilis GB519 GFP colonization dynamics rice blast control efficacy |
| 基金项目:吉林省农业科技创新工程(CXGC2021TD001, CXGC2021ZY018) |
| 作者 | 单位 | E-mail | | 祁山颜1, 2#, 朱 峰2#, 王继春2*, 田成丽2, 王东元1, 欧玉苹1, 2, 刘晓梅2, 李 莉2, 姜兆远2 | QI Shanyan1, 2#, ZHU Feng2#, WANG Jichun2*, TIAN Chengli2, WANG Dongyuan1, OU Yuping1, 2, LIU Xiaomei2, LI Li2, JIANG Zhaoyuan2 | wangjichun@cjaas.com |
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| 中文摘要: |
| 枯草芽胞杆菌GB519是一株具有广谱抑菌活性的生防菌株?本研究利用绿色荧光蛋白标记的菌株GB519-GFP处理水稻种子?根和叶片, 结合激光共聚焦显微镜观察和抗生素平板回收检测的方法, 探究其在水稻根茎叶中的定殖动态?结果显示:经GB519-GFP发酵液处理水稻种子?根和叶片后, 菌株均可内生定殖于植株的表皮?皮层和维管束中, 表明其可在水稻植株内迁移和定殖?GB519-GFP在处理部位的定殖量通常呈现先减少后增多的趋势, 非处理部位3~5 d后即可检测到标记菌株?浸种处理, 3 d后在幼芽中可检测到标记菌株; 20 d后在根中的菌量最多, 达5.7×105 cfu/g?灌根处理, 1 d后根中菌量为5.4×105 cfu/g; 20 d后根?茎和叶中菌量均达到最大值; 处理80 d后, 根中定殖数量仍达1.9×105 cfu/g?叶面喷施处理, 1 d后叶片菌量为4.2×105 cfu/g; 20 d后叶片菌量达4.4×105 cfu/g?不同处理方法在各部位的定殖量几乎均在处理20 d后达到峰值?采用叶面喷施GB519对稻瘟病穗颈瘟的田间防效达73.9%, 表明叶面喷施GB519能够起到防控稻瘟病的作用?本研究为使用枯草芽胞杆菌GB519防控水稻稻瘟病提供指导? |
| 英文摘要: |
| Bacillus subtilis GB519 displays antimicrobial activity in a broad spectrum. In this study, the GFP-labeled strain, GB519-GFP was used to explore its colonization dynamics in rice plants via both the laser confocal microscope observation and antibiotic plate recovery. The strain GB519-GFP could colonize the epidermis, cortex and vascular bundle after seed-soaking, root-irrigating or foliar-spray with the fermentation broth. These results indicated that it could migrate and colonize in rice plants. In general, the colonization quantity of GB519-GFP first decreased and then increased in the treated part, and it could be detected in the untreated part after 3-5 days. The GFP-labeled strain was detected in the bud three days after seed-soaking and the maximum amount of colonization reached 5.7×105 cfu/g in the root after 20 d. The amount of colonization in the root was 5.4×105 cfu/g one day after root-irrigating. The amount reached the maximum value in the root, stem and leaf after 20 d, and remained at 1.9×105 cfu/g in the root after 80 d. The colonization was 4.2×105 cfu/g in the leaves one day after foliar-spray, and reached 4.4×105 cfu/g after 20 d. The amounts of colonization in different tissues reached the peak 20 d after treated. The control efficacy of GB519 against rice blast was 73.9% in the field by foliar-spray. It indicated that foliar spraying with GB519 could play a role in preventing rice blast. This study provides a guidance for the control of rice blast by B. subtilis GB519. |
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