| 姚双艳, 张 耀, 杨 越, 舍泽龙, 王 焕, 卞会敏, 吴雪明, 魏纪珍, 安世恒*.Harelish 负向调控棉铃虫性信息素生物合成机制的初步研究[J].植物保护,2023,49(1):207-212. |
| Harelish 负向调控棉铃虫性信息素生物合成机制的初步研究 |
| Harelish negatively regulates sex pheromone biosynthesis in Helicoverpa armigera |
| 投稿时间：2022-08-08 修订日期：2022-09-27 |
| DOI：10.16688/j.zwbh.2022478 |
| 中文关键词: 棉铃虫 性信息素 Harelish 钙调磷酸酶 |
| 英文关键词:Helicoverpa armigera sex pheromone Harelish CaN |
| 基金项目:国家自然科学基金（31970472）；河南省现代农业产业技术体系（HARS-22-09-G3） |
| 作者 | 单位 | E-mail | | 姚双艳, 张 耀, 杨 越, 舍泽龙, 王 焕, 卞会敏, 吴雪明, 魏纪珍, 安世恒* | 小麦玉米作物学国家重点实验室, 河南省害虫绿色防控国际联合实验室, 河南农业大学植物保护学院, 郑州 450002 | anshiheng@aliyun.com |
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| 中文摘要: |
| 性信息素是蛾类昆虫通讯的关键信号分子, 其生物合成机制已在家蚕 Bombyx mori、棉铃虫 Helicoverpa armigera 和亚洲玉米螟 Ostrinia furnacalis 等蛾类中明确了, 但其负向调控机制尚不明确。本文探索了 Harelish 基因在棉铃虫雌蛾性信息素生物合成中的作用。结果发现, Harelish 基因在棉铃虫雌蛾中肠、表皮、脑、触角、脂肪体、肌肉和性信息素腺体等7个组织中均有表达, 且在羽化后随着性信息素生物的合成和释放而高丰度表达。基于RNAi降低 Harelish 基因转录水平的表达量后, 其性信息素含量和钙调磷酸酶活性均显著上升, 表明 Harelish 通过抑制钙调磷酸酶活性负向调控了性信息素的生物合成。该研究为利用性信息素生物合成的终止信号为分子靶标来防控害虫奠定理论基础。 |
| 英文摘要: |
| Sex pheromone is the key signal molecule in moth communication. The mechanism of sex biosynthesis has been elucidated in several moths, such as Bombyx mori, Helicoverpa armigera and Ostrinia furnacalis . However, the negative regulation mechanism is unknown. In the present study, Helicoverpa armigera was employed as a model insect to investigate the role of Harelish in sex pheromone biosynthesis. The results showed that the Harelish gene was ubiquitously expressed in seven tissues of female moth, including midgut, epidermis, brain, antenna, fat body, muscle and sex pheromone gland. The developmental expression profiling showed that Harelish was highly expressed during the biosynthesis and release of sex pheromone. RNAi-mediated knockdown of Harelish led to significant decrease in Harelish transcripts. After successful knockdown of Harelish, the decrease of Harelish transcripts caused a significant increase in calcineurin activity and sex pheromone production, suggesting that Harelish negatively regulates sex pheromone biosynthesis by inhibiting CaN activity in H. armigera. These results provide a theoretical foundation for using the termination signal of sex pheromone biosynthesis as a molecular target to control pests. |
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