| 吴思雨1, 2#, 梁 赫3#, 刘 秀2, 路 伟3, 邵雪花1*.没食子酸对棉铃虫生长发育的影响及作用机理[J].植物保护,2023,49(1):118-125. |
| 没食子酸对棉铃虫生长发育的影响及作用机理 |
| Effect and mechanism of gallic acid on growth and development of Helicoverpa armigera |
| 投稿时间：2022-08-02 修订日期：2022-08-29 |
| DOI：10.16688/j.zwbh.2022466 |
| 中文关键词: 没食子酸 棉铃虫 生长发育 营养调控 中肠细胞凋亡 |
| 英文关键词:gallic acid Helicoverpa armigera growth and development nutritional regulation midgut cell apoptosis |
| 基金项目:新疆维吾尔自治区重点研发专项(2022B02033-1); 新疆农业科学院科技创新重点培育专项(xjkcpy-2020004) |
| 作者 | 单位 | E-mail | | 吴思雨1, 2#, 梁 赫3#, 刘 秀2, 路 伟3, 邵雪花1* | 1. 广东省农业科学院果树研究所, 农业农村部南亚热带果树生物学与遗传资源利用重点实验室, 广东省热带亚热带果树研究重点实验室, 广州 510640；2. 湖南人文科技学院, 农作物有害生物绿色防控湖南省高校重点实验室, 娄底 417000
3. 新疆农业大学农学院, 乌鲁木齐 830052 | sxh19831017@163.com |
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| 中文摘要: |
| 为明确没食子酸(GA)对棉铃虫生长发育的影响和作用机理, 用叶碟法对比了没食子酸等21个酚类化合物对棉铃虫幼虫的拒食活性, 并从中选择了没食子酸等3种化合物测定了其对棉铃虫1龄末幼虫的拒食中浓度(AFC50), 随后采用饲料混毒法检测了没食子酸对棉铃虫生长发育的影响, 进一步测定了2龄幼虫中肠病理变化和生长发育相关基因的表达变化。结果表明：所测21种酚类化合物中, 没食子酸对1龄末幼虫的拒食活性最显著, 拒食中浓度为50.21 mg/L。以含10 μg/g没食子酸的饲料饲喂棉铃虫幼虫后发现其显著抑制幼虫体重、化蛹率、羽化率和卵孵化率。苏木精—伊红染色法检测到没食子酸可诱导中肠细胞大量凋亡、杯状细胞和消化细胞膨大并呈现空泡化、围食膜消失等病理变化；qPCR分析发现没食子酸可诱导 InR、p53、Atg8 和 G6Pase 基因显著上调表达。综上, 没食子酸可显著抑制棉铃虫的生长发育, 同时损伤中肠, 导致营养代谢紊乱, 具备开发成为新型植物源杀虫剂的潜力。 |
| 英文摘要: |
| In order to clarify the effect and mechanism of gallic acid (GA) on the growth and development of Helicoverpa armigera, the antifeedant activity of 21 phenolic compounds including GA on H. armigera larvae and the antifeedant medium concentration(AFC50) of three compounds including GA were compared by leaf disc method. Then, the effect of GA on the growth and development of H. armigera was detected by mixing feed-compound, and the pathological changes in the midgut and the expression changes of growth and development-related genes of the 2nd-instar larvae were further analyzed. The results showed that among the 21 phenolic compounds tested, GA had the significantly antifeedant activity against the 1st-instar larvae, and AFC50 was 50.21 mg/L; after feeding the larvae of H. armigera with the feed containing 10 μg/g GA, the larval weight, pupation rate and eclosion, and egg hatching rate were significantly inhibited. The results of hematoxylin-eosin staining revealed that GA could induce massive apoptosis of midgut cells, vacuolization of digestive cells, disappearance of peritrophic membrane. The qPCR analysis found that GA could induce significant upregulation of InR, p53, Atg 8 and G6Pase genes. In conclusion, GA can significantly inhibit the growth and development of H. armigera, and damage the midgut, lead to nutritional metabolism disorder, and has the potential to be developed into a new type of plant-derived insecticide. |
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