| 陈夕军*, 石 童, 陈 宸, 唐 滔.喷施诱导的基因沉默(SIGS)技术控制植物病害研究进展[J].植物保护,2022,48(5):15-22. |
| 喷施诱导的基因沉默(SIGS)技术控制植物病害研究进展 |
| Advances in the control of plant diseases via spray-induced gene silencing |
| 投稿时间：2021-08-31 修订日期：2021-11-11 |
| DOI：10.16688/j.zwbh.2021477 |
| 中文关键词: 喷施诱导的基因沉默 双链RNA RNA干扰 真菌病害 |
| 英文关键词:spray-induced gene silencing dsRNA RNA interference fungal disease |
| 基金项目:江苏省重点研发计划(现代农业)重点项目(BE2020319); 江苏省现代农业产业发展项目(2020-SJ-003-YD07) |
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| 中文摘要: |
| 喷施诱导的基因沉默(spray-induced gene silencing, SIGS)技术是一种新型基因沉默技术, 其以病原菌生长发育和致病相关基因为靶标, 将体外合成的针对靶基因的dsRNA喷施于植物表面, 抑制靶基因的表达?在病原菌侵染寄主的过程中, 病原菌可直接从寄主植物表面摄取dsRNA, 也可由植物吸收dsRNA后, 直接以dsRNA的形式或将dsRNA剪切成sRNAs转运至病原菌体内诱导病原菌相关基因沉默, 从而抑制病菌的侵染和扩展?由于SIGS技术不需要转基因, 再加上其具有高效性?特异性和环境友好性, 故显示出巨大的应用潜力?本文综述了SIGS技术的最新研究进展, 总结了影响SIGS控制植物病害的因素及未来的研究方向, 展望了其应用前景? |
| 英文摘要: |
| Spray-induced gene silencing (SIGS) is a novel RNAi method inducing gene silencing. It targets the genes related to the growth, development and pathogenicity of pathogens and sprays dsRNA synthesized in vitro against the target gene on the plant surface to inhibit the expression of the target gene. In the course of infection, pathogens can directly take up dsRNA molecules from the plant surface, and these dsRNA molecules can also be taken up by plants, even cleaved into sRNAs, and then transferred into pathogens to interfere the expression of the target genes and inhibit the infection and expansion of the pathogens. SIGS shows great application potential because of its high efficiency, specificity, environmental friendliness, without genetically modified products. In this review, recent advances in SIGS were summarized, and the factors influencing plant disease control, future research directions and application prospects of SIGS were also discussed. |
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