崔一平, 彭埃天*, 宋晓兵, 陈 霞, 程保平, 凌金锋.广东省江门市‘新会柑’叶斑病病原菌鉴定[J].植物保护,2022,48(4):236-239. |
广东省江门市‘新会柑’叶斑病病原菌鉴定 |
Identification of the pathogen causing leaf spot on Citrus reticulata ‘Chachiensis’ in Jiangmen city of Guangdong province |
投稿时间:2021-05-25 修订日期:2021-06-28 |
DOI:10.16688/j.zwbh.2021311 |
中文关键词: 新会柑 叶斑病 多基因系统发育 番茄匍柄霉 |
英文关键词:Citrus reticulata ‘Chachiensis’ leaf spot multi-gene phylogeny Stemphylium lycopersici |
基金项目:科技创新战略专项资金(R2018QD-059);广东省现代农业产业技术体系建设项目(2018LM1077) |
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中文摘要: |
近年来广东省江门市‘新会柑’上出现一种叶斑病, 症状表现为叶尖和叶缘出现不规则的、带有黄色晕圈的棕褐色斑点。为明确引起该病的病原菌, 作者利用组织分离法对病样进行分离纯化, 采用ITS序列和LSU基因进行系统发育学分析, 同时结合形态特征观察对纯化后的菌株进行鉴定, 结合柯赫氏法则对其进行验证。结果确定病原菌为番茄匍柄霉Stemphylium lycopersici。 |
英文摘要: |
In recent years, a Citrus reticulata ‘Chachiensis’ leaf spot disease appeared in Jiangmen city, Guangdong province. This disease mostly occurs on young leaves, and the symptoms are irregular brown spots with yellow halos on leaf tips and leaf margins. Based on tissue isolation, multi-sequence phylogenetic analysis (ITS and LSU), morphological characters and Koch’s postulates, the pathogen was identified as Stemphylium lycopersici. |
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