| 凌 彬1, 2, 纪 颖2, 3, 白 云1, 2, 朱家君2, 3, 陈思宇1, 2, 李紫溢2, 3, 段 可2*, 高清华2*.UV-C照射对草莓炭疽病3种致病菌生活力和致病力的影响[J].植物保护,2022,48(4):203-210. |
| UV-C照射对草莓炭疽病3种致病菌生活力和致病力的影响 |
| Influences of ultraviolet C radiation on the viability and pathogenicity of three Colletotrichum spp. causing strawberry anthracnose |
| 投稿时间：2021-05-31 修订日期：2021-07-22 |
| DOI：10.16688/j.zwbh.2021322 |
| 中文关键词: 草莓炭疽病 胶孢炭疽菌复合种 分生孢子 UV-C辐照 |
| 英文关键词:strawberry anthracnose Colletotrichum gloeosporioides sensu lato conidia ultraviolet C radiation |
| 基金项目:上海市科技兴农项目(2019-02-08-00-08-F01108) |
| 作者 | 单位 | E-mail | | 凌 彬1, 2, 纪 颖2, 3, 白 云1, 2, 朱家君2, 3, 陈思宇1, 2, 李紫溢2, 3, 段 可2*, 高清华2* | 1. 上海海洋大学食品学院, 上海 201306
2. 上海市农业科学院林木果树研究所, 上海 201403
3. 上海应用技术大学生态技术与工程学院, 上海 201418 | 段可kduan936@126.com; 高清华qhgao20338@sina.com |
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| 中文摘要: |
| 炭疽病是草莓种植中普遍发生、危害严重的病害之一, 而UV-C对真菌的生长繁殖有极大的影响。为了探究UV-C辐照对引起草莓炭疽病的3种主要病原菌生长和致病力影响的差异, 以果生炭疽菌Colletotrichum fructicola、胶孢炭疽菌C.gloeosporioides和暹罗炭疽菌C.siamense为供试菌, 分析UV-C辐照对其分生孢子存活、菌丝生长和产孢能力及对草莓叶片致病力的影响。结果显示, 在105~420 J/m2辐照剂量（辐照30~120 s）范围内, 3种炭疽菌孢子的相对存活率对UV-C辐照剂量的响应存在显著差异, C.gloeosporioides的耐受性最强, C.fructicola最为敏感, 辐照剂量420 J/m2(辐照120 s)下, 3种炭疽菌孢子的相对存活率在4%或以下, 接近分生孢子的致死剂量。840~1 260 J/m2辐照剂量（辐照4~6 min）下, C.fructicola与C.gloeosporioides菌丝生长对UV-C辐照的耐受性相当; 1 680~2 520 J/m2辐照剂量（辐照8~12 min）下, 3种炭疽菌对不同时间的UV-C辐照的耐受性存在差异。C.gloeosporioides菌丝产孢对UV-C辐照最敏感, C.siamense最耐受, C.fructicola在辐照剂量1 260 J/m2(辐照6 min)时产孢量较辐照2 min和4 min明显上升。3种炭疽病菌的致病力与其对UV-C的耐受性没有明显相关性。本研究为我国草莓炭疽病的非化学农药控制探索了新途径。 |
| 英文摘要: |
| Anthracnose is one of the main diseases that commonly occur and cause serious damage in strawberry planting, and ultraviolet C(UV-C) has a great influence on the growth and reproduction of fungi. To explore the differences in the influences of UV-C irradiation on the growth and pathogenicity of three dominant pathogens causing strawberry anthracnose, Colletotrichum fructicola, C.gloeosporioides and C.siamense were used to analyze their responses to UV-C irradiation in conidial viability, colony development, hyphal sporulation ability, and pathogenicity to strawberry after exposure to UV-C radiation. The results showed that, when the dose of UV-C irradiation ranged between 105-420 J/m2 (irradiation duration: 30-120 s), there was a significant difference in the relative survival rate of conidia among three Colletotrichum species. C.gloeosporioides was most tolerant to UV-C irradiation, and C. fructicola was most sensitive. The relative survival rate of conidia of three species was less than 4% after UV-C irradiation at a dose of 420 J/m2 (irradiation duration: 120 s), approximately a lethal dose for conidia. When the dose of UV-C radiation was between 840-1 260 J/m2 (irradiation duration: 4-6 min), C.fructicola and C.gloeosporioides displayed similar tolerance to UV-C in colony development. However, under higher irradiation doses of 1 680-2 520 J/m2 （irradiation duration: 8-12 min）, these Colletotrichum species varied greatly in colony development. As for the ability of hyphal sporulation, C.gloeosporioides was most sensitive to UV-C irradiation; C.siamense was most tolerant, and the sporulation of C.fructicola had a obvious recovery after irradiation at a dose of 1 260 J/m2 (irradiation duration： 6 min). The pathogenicity of Colletotrichum species was not significantly related to their tolerance to UV-C irradiation. This study provides some clues for exploring novel approaches for non-chemical control of strawberry anthracnose in the future. |
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