| 高国龙, 张兴旺, 姜子健, 黄家风*, 都业娟*.木尔坦棉花曲叶病毒在新疆的发生分布及烟粉虱带毒检测[J].植物保护,2022,48(3):254-262. |
| 木尔坦棉花曲叶病毒在新疆的发生分布及烟粉虱带毒检测 |
| Distribution of cotton leaf curl Multan virus and detection of viruliferous rate of Bemisia tabaci in Xinjiang |
| 投稿时间：2021-03-12 修订日期：2021-04-25 |
| DOI：10.16688/j.zwbh.2021154 |
| 中文关键词: CLCuMuV 棉花 蜀葵 苘麻 烟粉虱 |
| 英文关键词:CLCuMuV cotton Alcea rosea Abutilon theophrasti Bemisia tabaci |
| 基金项目:国家自然科学基金（31860496）；新疆生产建设兵团区域创新引导计划（2018BB403）；国家大学生创新创业训练计划（202010759002） |
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| 中文摘要: |
| 为了明确木尔坦棉花曲叶病毒cotton leaf curl Multan virus (CLCuMuV)在新疆的发生分布,对采自新疆13个地(州)的1 166份蜀葵、苘麻、棉花疑似病株进行CLCuMuV分子鉴定和带毒检测,并对3种寄主上同期采集的1 588头烟粉虱进行隐种鉴定和带毒检测。结果显示,CLCuMuV DNA-A组分及其伴随的DNAβ卫星分子均可侵染蜀葵、苘麻、棉花;基于DNA-A及DNAβ组分的进化分析表明,新疆3种寄主上发生的CLCuMuV与国内已报道的CLCuMuV 进化关系一致;蜀葵、苘麻、棉花上CLCuMuV带毒率存在差异,分别为27.87%、33.33%和14.95%;MEAM1和MED隐种为新疆烟粉虱主要种群,分别占26.70%和66.44%,带毒率分别为21.46%和17.63%,说明MED隐种为新疆烟粉虱优势种群, 2个隐种均可携带CLCuMuV且带毒率相近;MEAM1隐种在蜀葵、苘麻、棉花上带毒率分别为21.39%、0和28.21%,MED隐种分别为19.61%,8.47%和11.18%,表明2个隐种在不同寄主上的带毒率存在差异。本研究明确了新疆地区侵染蜀葵、苘麻和棉花的CLCuMuV发生及分布情况, 并明确新疆烟粉虱主要种群MEAM1和MED 隐种在3种寄主上的分布及带毒情况,为新疆棉区防控CLCuMuV发生严重危害提供科学依据。 |
| 英文摘要: |
| In order to determine the distribution of cotton leaf curl Multan virus (CLCuMuV) in Xinjiang, 1 166 suspected plants of Alcea rosea, Abutilon theophrasti and cotton collected from 13 prefectures (autonomous prefectures) in Xinjiang were investigated by molecular identification and CLCuMuV detection, and the total of 1 588 Bemisia tabaci were also collected from the three hosts to determine the cryptic species of B. tabaci and their viruliferous rate of CLCuMuV by PCR. The results showed that A. rosea, A. theophrasti and cotton could be infected by CLCuMuV DNA-A and its beta satellite DNAβ. CLCuMuV DNA-A and its DNAβ from the three hosts in Xinjiang were closely related to the CLCuMuV isolates from other provinces in China based on phylogenetic tree analysis. The rate of CLCuMuV infection in A. rosea, A. theophrasti and cotton was 27.87%, 33.33% and 14.95%, respectively. The population of B. tabaci in Xinjiang was mainly comprised of MEAM1 and MED cryptic species. MEAM1 accounted for 26.70% with 21.46% viruliferous rate, and MED accounted for 66.44% with 17.63% viruliferous rate, indicating that MED cryptic species is the dominant population of B. tabaci in Xinjiang. Both MEAM1 and MED could carry CLCuMuV with similar viruliferous rate, the viruliferous rate of MEAM1 in A. rosea, A. theophrasti and cotton was 21.39%, 0 and 28.21%, respectively, and that of MED was 19.61%, 8.47% and 11.18%, respectively. The results showed that the viruliferous rates of MEAM1 and MED on different hosts were different. In conclusion, the occurrence and distribution of CLCuMuV in A. rosea, A. theophrasti and cotton in Xinjiang were determined, and the distribution and viruliferous rates of MEAM1 and MED on the three hosts were also determined, which provides scientific basis for the prevention and control of CLCuMuV in Xinjiang. |
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