| 惠娜娜1, 2, 王 立1, 2, 李继平1, 2, 3*, 郑 果1, 2, 李培玲1, 马生彪3, 吕昭龙3.甘肃省部分地区马铃薯晚疫病菌交配型及线粒体DNA单倍型分析[J].植物保护,2022,48(2):177-182. |
| 甘肃省部分地区马铃薯晚疫病菌交配型及线粒体DNA单倍型分析 |
| Analysis of mating type and mitochondrial DNA haplotypes of Phytophthora infestans collected from part areas in Gansu province |
| 投稿时间：2021-01-18 修订日期：2021-05-07 |
| DOI：10.16688/j.zwbh.2021037 |
| 中文关键词: 马铃薯 晚疫病菌 交配型 线粒体DNA单倍型 |
| 英文关键词:potato Phytophthora infestans mating type mtDNA haplotype |
| 基金项目:国家自然科学基金(31560487); 甘肃省重点研发计划(18YF1NA095); 甘肃省农业科学院重点研发计划(2019GAAS23); 甘肃省民生科技专项(20CX9NA091); 国家马铃薯标准化区域服务与推广平台项目(NBFW-17-2019)? |
| 作者 | 单位 | E-mail | | 惠娜娜1, 2, 王 立1, 2, 李继平1, 2, 3*, 郑 果1, 2, 李培玲1, 马生彪3, 吕昭龙3 | 1. 甘肃省农业科学院植物保护研究所, 兰州 730070
2. 农业农村部天水作物有害生物科学观测实验站, 天水 741200
3. 甘肃农业大学植物保护学院, 兰州 730070 | gslijp@163.com |
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| 中文摘要: |
| 由致病疫霉Phytophthora infestans (Mont.) de Bary侵染引起的马铃薯晚疫病是一种世界范围内的毁灭性病害?为了明确甘肃省马铃薯晚疫病菌的遗传结构, 对2018年甘肃省定西市?张掖市和陇南市采集的130株晚疫病菌进行了交配型和线粒体DNA (mtDNA)单倍型测定?结果表明:从130株晚疫病菌中检测到A1和A2两种交配型, A1交配型为14株(10.77%), A2交配型为116株(89.23%); 检测到Ⅰa?Ⅱa和Ⅱb 3种线粒体 DNA单倍型, 发生频率分别为88.46%?3.85%和7.69%?Ⅰa单倍型菌株的交配型为A1和A2, Ⅱa单倍型菌株交配型为A1和A2, Ⅱb单倍型菌株交配型为A1?该结果表明甘肃省部分地区马铃薯晚疫病菌以A2交配型, Ⅰa mtDNA单倍型为主?甘肃省各采集地晚疫病菌群体结构或与当地种薯繁育?调运有关? |
| 英文摘要: |
| Potato late blight caused by Phytophthora infestans (Mont.) de Bary is a destructive disease in the world. In order to clarify the genetic structure of P.infestans in Gansu province, the mating type and mtDNA haplotype of 130 P.infestans strains collected in Dingxi city, Zhangye city and Longnan city in 2018 were determined. The results showed that A1 and A2 mating types were detected from 130 strains, among which 14 strains (10.77%) were the A1 mating type and 116 strains (89.23%) were A2. Three mtDNA haplotypes of Ⅰa, Ⅱa and Ⅱb were detected from 130 strains, and the occurrence frequencies were 88.46%, 3.85% and 7.69%, respectively. The mating types of Ⅰa haplotype strains were A1 and A2, Ⅱa haplotype strains were A1 and A2, and Ⅱb haplotype strains were A1. The results showed that the main pathogens of P.infestans in Gansu province were A2 mating type and Ⅰa mtDNA haplotype. The population structure of P.infestans in different collection sites in Gansu province may be related to the breeding and transportation of local seed potatoes. |
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