| 林艾佳#, 吴 奇#, 倪瑞莲, 陈 恒, 卫 冕, 李成云 *, 王 一*.钙离子响应蛋白GCaMP6s在稻瘟菌中的异源表达[J].植物保护,2022,48(2):9-15. |
| 钙离子响应蛋白GCaMP6s在稻瘟菌中的异源表达 |
| Heterologous expression of Ca2+ sensor protein GCaMP6s in Magnaporthe oryzae |
| 投稿时间：2021-01-11 修订日期：2021-02-01 |
| DOI：10.16688/j.zwbh.2021023 |
| 中文关键词: 稻瘟菌 异源表达 GCaMP6s |
| 英文关键词:Magnaporthe oryzae heterologous expression GCaMP6s |
| 基金项目:国家重点研发计划(2017YFD0200400); 云南省基础研究计划青年基金(202101AU070101); 云南省教育厅科学研究基金(2020J0252); 省部共建云南生物资源利用与保护国家重点实验室科学研究基金(GZJS201903) |
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| 中文摘要: |
| 稻瘟病严重威胁全球水稻的产量, 钙离子信号通路参与了稻瘟菌生长?发育和致病, 了解这些过程中菌株细胞质中钙离子浓度的变化对稻瘟病的防治具有重要意义?GCaMP6s作为一种细胞质钙离子感受蛋白在动物和植物中已有大量应用?本研究利用不同稻瘟菌菌株构建了GCaMP6s基因的异源表达菌株, 在0.01 g/mL钙离子处理下, GCaMP6s在稻瘟菌菌丝和分生孢子中稳定表达?此外, GCaMP6s异源表达菌株与野生型菌株的菌落直径和产孢量没有差异, 表明GCaMP6s基因的异源表达不影响稻瘟菌的菌丝生长和产孢?本研究获得了GCaMP6s异源表达的稻瘟菌菌株, 为后续研究稻瘟菌细胞质中钙离子浓度变化与菌株生长发育和胁迫响应提供研究材料? |
| 英文摘要: |
| Rice blast caused by Magnaporthe oryzae is a serious threat to the global rice production. Calcium signaling pathway is involved in the growth, development and pathogenicity of M. oryzae. Understanding the changes of Ca2+ concentration in the cytoplasm of M. oryzae at three different stages is of great significance for control of rice blast. As a cytoplasmic Ca2+ sensor protein, GCaMP6s has been widely used in animals and plants. In this study, heterologous expression strains containing GCaMP6s were constructed using M. oryzae YN153 and YN157 strains. Under 0.01 g/mL Ca2+ treatment, GCaMP6s was stably expressed in the mycelia and conidia of M. oryzae. In addition, by comparing the colony diameter and sporulation between the heterologous expression strains of GCaMP6s and the wild type strains, we found that there was no difference between the two strains, indicating that the heterologous expression of GCaMP6s gene did not affect the mycelial growth and sporulation of M. oryzae. In this study, we obtained the heterologous expression of GCaMP6s in M. oryzae, which can provide research materials for further study on the changes of calcium ion concentration in the cytoplasm, and the growth, development and stress response of M. oryzae. |
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