| 陈新亮, 房伯平, 王章英, 姚祝芳, 杨义伶, 黄立飞*.国家种质广州甘薯圃病毒种类鉴定及分析[J].植物保护,2022,48(1):258-264. |
| 国家种质广州甘薯圃病毒种类鉴定及分析 |
| Identification and analysis of viruses in the national germplasm nursery for sweet potato(Guangzhou) |
| 投稿时间：2020-10-22 修订日期：2021-01-07 |
| DOI：10.16688/j.zwbh.2020557 |
| 中文关键词: 甘薯 种质资源 病毒病 复合侵染 |
| 英文关键词:sweet potato germplasm resources virus disease co infection |
| 基金项目:国家农作物种质资源保护与利用项目(NWB014);国家现代农业(甘薯)产业技术体系建设专项(CARS-10-B-5, CARS-10-C-17);广东省现代农业产业技术体系甘薯马铃薯创新团队(2019KJ111) |
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| 中文摘要: |
| 为明确引起国家种质广州甘薯资源圃中病毒病的病毒种类及优势种, 为甘薯种质安全保存提供支持，2017年从甘薯资源圃中未脱毒更新的盆栽苗和大田苗中采集155份具有不同病毒病症状的甘薯资源样品, 利用PCR和RT PCR检测技术对这些样品进行了17种病毒的分子检测。155份样品均有病毒检出, 包括甘薯羽状斑驳病毒 Sweet potato feathery mottle virus (SPFMV)、甘薯褪绿矮化病毒 Sweet potato chlorotic stunt virus (SPCSV)、黄瓜花叶病毒 Cucumber mosaic virus (CMV)、甘薯褪绿斑病毒 Sweet potato chlorotic fleck virus (SPCFV)、甘薯类花椰菜花叶病毒Sweet potato caulimo like virus(SPCV)、甘薯潜隐病毒 Sweet potato latent virus (SPLV)、甘薯脉花叶病毒Sweet potato vein mosaic virus (SPVMV)、甘薯病毒2 Sweet potato virus 2 (SPV2)和甘薯曲叶病毒 Sweet potato leaf curl virus (SPLCV)等9种病毒。检出率最高的是SPCFV, 为98.1%, 最低的是SPCV, 为0.6%;RNA病毒检出率为99.4%, DNA病毒检出率为94.2%。154份样品为病毒复合侵染, 占总样品数的99.4%;其中4~6种病毒复合侵染率最高, 为70.7%, 有4份样品最多检测出8种病毒(2.6%)。 在所有复合侵染类型中, 同时含有SPFMV和SPCSV两种病毒的复合侵染率占54.2%。研究结果表明, 甘薯种质资源经过多年茎蔓和种薯繁育, 病毒感染率高, 病毒种类多, 以复合侵染为主, 在生长环境相同的条件下, 不同资源品种对病毒的耐受情况存在明显差异。 |
| 英文摘要: |
| In order to investigate the virus and dominant virus species causing sweet potato virus disease in the national sweet potato germplasm Guangzhou resource nursery, provide support for the safe preservation of resources, a total of 155 samples with viral symptoms were collected from potted seedlings and field seedlings in nursery in 2017. Molecular detection of viruses in the samples were carried out by PCR or RT PCR using primers for seventeen viruses. The results showed that nine virus species were detected, including Sweet potato feathery mottle virus (SPFMV), Sweet potato chlorotic stunt virus (SPCSV), Cucumber mosaic virus (CMV), Sweet potato chlorotic fleck virus (SPCFV), Sweet potato caulimo like virus (SPCV), Sweet potato latent virus (SPLV), Sweet potato vein mosaic virus (SPVMV), Sweet potato virus 2 (SPV2) and Sweet potato leaf curl virus (SPLCV). Among which, SPCFV had the highest detection rate of 98.1%, and SPCV had the lowest detection rate of 0.6%. The detection rate of RNA viruses was 99.4%, and that of DNA viruses was 94.2%. One hundred and fifty four samples (99.4%) were found to be infected by two or above viruses. Specifically, co infection by 4-6 kinds of viruses were major type, accounting for 70.7%. A total of eight viruses were detected in four samples (2.6%). Combined infection contained SPFMV and SPCSV was the most common, accounting for 54.2%. In short, some germplasm resources of sweet potato have been infected by different viruses with high infection rates and mixed infection was dominant. The tolerance of different resources to the virus is obviously different under the same growth environment. |
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