| 庞继鑫1#, 温绍海1#, 杜广祖1, 杨 航2, 马 芸1, 杨进波2, 付玉飞1, 张庭发2, 易小光2, 李正跃1*, 陈 斌1*.一株侵染草地贪夜蛾成虫的球孢白僵菌的分离鉴定[J].植物保护,2022,48(1):185-190. |
| 一株侵染草地贪夜蛾成虫的球孢白僵菌的分离鉴定 |
| Isolation and identification of an entomopathogenic fungus infecting the adult of Spodoptera frugiperda (J. E. Smith) |
| 投稿时间：2020-11-04 修订日期：2021-01-07 |
| DOI：10.16688/j.zwbh.2020587 |
| 中文关键词: 球孢白僵菌 草地贪夜蛾 昆虫病原真菌 致病力 |
| 英文关键词:Beauveria bassiana Spodoptera frugiperda entomopathogenic fungus pathogenicity |
| 基金项目:云南省重点领域科技计划(202001BB050002); 国家重点研发计划(2019YFD0300105) |
| 作者 | 单位 | E-mail | | 庞继鑫1#, 温绍海1#, 杜广祖1, 杨 航2, 马 芸1, 杨进波2, 付玉飞1, 张庭发2, 易小光2, 李正跃1*, 陈 斌1* | 1. 云南农业大学植物保护学院, 云南生物资源保护与利用国家重点实验室, 昆明 650201
2. 云南格瑞生物科技有限公司, 大理 675600 | 李正跃 kmlizhengyue@163.com; 陈斌 chbins@163. com |
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| 中文摘要: |
| 草地贪夜蛾 Spodoptera frugiperda (J. E. Smith)是一种全球性重大玉米害虫, 于2019年入侵我国云南。2020年8月, 从云南省大理弥渡西庄村玉米田采集被虫生真菌感染的草地贪夜蛾雌成虫, 经在室内分离培养, 结合病原菌分生孢子与菌丝等形态及ITS序列相似性分析, 确定感染草地贪夜蛾成虫的真菌为球孢白僵菌 Beauveria bassiana, 菌株编号为SHL-2020。经生物测定, 该菌对草地贪夜蛾雌、雄成虫均具有较强的侵染致病作用。用该菌1×108个/mL 分生孢子菌悬液接种处理后第7天时，草地贪夜蛾雌、雄成虫累计死亡率分别为90.0%和86.7%。以上结果表明, 从罹病草地贪夜蛾雌成虫上分离获得的球孢白僵菌SHL-2020菌株对草地贪夜蛾雌雄成虫具有良好的生防潜力, 该研究为草地贪夜蛾生物防治提供了理论依据和物质基础。 |
| 英文摘要: |
| Spodoptera frugiperda (J. E. Smith) is a global major pest and invaded into Yunnan province in 2019. An infected female adult of S.frugiperda was found in corn planting field in Xizhuang village in Midu county of Dali city, Yunnan province in August 2020, and was identified by combining morphology and ITS sequence analysis. The pathogenicity of the fungus to male and female adult of the S.frugiperda was determined by conidia suspension spray method. The results indicated the fungus was identified to be Beauveria bassiana, which was named as SHL-2020. The fungus was pathogenic to both male and female adults, and the cumulative adjusted mortality was 86.7% and 90.0% at the 7th day after infection when the spore concentration was 1×108 spore/mL. The above results indicated that B.bassiana SHL-2020 fungus isolated from the female adult S.frugiperda has good biocontrol potentiality to the male and female adults of S. frugiperda, which provide the theoretical and material base for the development of the biocontrol method for S. frugiperda . |
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