| 张春雨1, 李小宇1, 王 晨1, 2, 战笑蕾1, 3, 刘建青1, 3, 王永志1*.侵染大豆的花生斑驳病毒公主岭分离物基因组测序及分析[J].植物保护,2022,48(1):61-65. |
| 侵染大豆的花生斑驳病毒公主岭分离物基因组测序及分析 |
| Sequencing and analysis of the genome of Peanut mottle virus (PeMoV) Gongzhuling isolate infecting soybean |
| 投稿时间：2020-10-16 修订日期：2020-11-24 |
| DOI：10.16688/j.zwbh.2020544 |
| 中文关键词: 花生斑驳病毒 基因组 大豆 |
| 英文关键词:Peanut mottle virus (PeMoV) genome soybean |
| 基金项目:吉林省科技厅重点研发计划(20180201013NY)；吉林省农业科技创新工程(CXGC2021ZY022) |
| 作者 | 单位 | E-mail | | 张春雨1, 李小宇1, 王 晨1, 2, 战笑蕾1, 3, 刘建青1, 3, 王永志1* | 1. 吉林省农业科学院, 公主岭 136100
2. 吉林农业大学, 长春 130118
3. 长春师范大学, 长春 130032 | yzwang@126.com |
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| 中文摘要: |
| 本研究利用小RNA深度测序法在大豆叶片上检测到1株花生斑驳病毒 Peanut mottle virus (PeMoV), 根据小RNA深度测序结果和GenBank公布的PeMoV基因组序列设计引物克隆了PeMoV公主岭分离物(PeMoV Gongzhuling)的基因组序列。测序结果经拼接后获得了PeMoV Gongzhuling基因组, 大小为9 709个核苷酸(GenBank登录号:MT790744)。该基因组在第123位—第9 422位存在1个大的开放阅读框(ORF), 编码1个多聚蛋白(分子量351.28 kD)。PeMoV Gongzhuling与GenBank中已登录的其他PeMoV分离物的基因组的核苷酸序列一致性为95.88%~99.53%, 氨基酸序列一致性为97.39%~99.84%, 其中与韩国分离物GYBU 92 (MT603819)在核苷酸和氨基酸水平上的一致性均为最高。 |
| 英文摘要: |
| In this study, the Peanut mottle virus (PeMoV) from soybean leaves was detected by small RNA deep sequencing. Primers were designed according to results of sRNA deep sequencing and the published genome sequences of PeMoV on GenBank, and the segments of genome of PeMoV Gongzhuling isolate (PeMoV Gongzhuling) was amplified by RT PCR. The assembled genome of PeMoV Gongzhuling was 9 709 nucleotides (GenBank accession no. MT790744). A large open reading frame (ORF) was found located between the 123th and the 9422th nucleotides, which encoded a polypeptide of 351.28 kD. By comparing it with other isolates of PeMoV, the nucleotide identity was 95.88%-99.53%, and the amino acid identity was 97.39%-99.84%, and it shared the highest identity with the South Korea isolate GYBU 92 (MT603819). |
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