| 孙东晗1, 陈悦1, 田文学2, 白庆荣1*, 赵廷昌3*.吉林省西瓜蔓枯病菌对咪鲜胺和苯醚甲环唑敏感基线的建立和抗性监测[J].植物保护,2021,47(6):260-264. |
| 吉林省西瓜蔓枯病菌对咪鲜胺和苯醚甲环唑敏感基线的建立和抗性监测 |
| Establishment of baseline sensitivity and monitoring of resistance of Stagonosporopsis citrulli on watermelon to prochloraz and difenoconazole in Jilin province |
| 投稿时间：2020-09-16 修订日期：2020-10-08 |
| DOI：10.16688/j.zwbh.2020494 |
| 中文关键词: 西瓜蔓枯病菌 抗药性 咪鲜胺 苯醚甲环唑 |
| 英文关键词:Stagonosporopsis citrulli fungicide resistance prochloraz difenoconazole |
| 基金项目:国家重点研发计划（2018YFD0201300）; 国家西甜瓜产业技术体系（CARS-25） |
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| 中文摘要: |
| 为明确吉林省西瓜蔓枯病菌Stagonosporopsis citrulli对咪鲜胺和苯醚甲环唑的抗药性水平, 本试验采用菌丝生长速率法测定了151株西瓜蔓枯病菌对这两种药剂的敏感性及两种药剂对西瓜蔓枯病菌的MIC值, 并测定了吉林省7个西瓜主产区的375株菌株对这两种药剂的抗性水平。结果表明, 供试的151株菌株对这两种药剂的敏感性频率分布均呈连续的单峰曲线, 接近正态分布; 对咪鲜胺和苯醚甲环唑的敏感基线分别为(0.203 0±0.135 3) μg/mL和(0.675 9±0.394 2) μg/mL; 两种药剂的最低抑制浓度MIC分别为20 μg/mL和40 μg/mL。供试375株菌株对两种杀菌剂的抗性频率均为0。本研究结果为咪鲜胺和苯醚甲环唑的合理用药策略提供了科学依据, 为后续其抗性风险评估奠定了基础。 |
| 英文摘要: |
| To evaluate the resistance levels of Stagonosporopsis citrulli causing gummy stem blight of Citrullus lanatus in Jinlin province to prochloraz and difenoconazole, the baseline sensitivities of S.citrulli strains (n=151) to the two fungicides and the minimum inhibitory concentrations (MICs) of the two fungicides to S.citrulli strains were determined by using mycelial growth rate method. The resistance levels of 375 stains from seven main watermelon production areas of Jinlin province to two fungicides were also determined. The results showed that the sensitivity frequency distribution of tested strains(n=151) to prochloraz and difenoconazole presented continuous single peak curves, which were close to normal distribution. The baseline sensitivities of S.citrulli strains to prochloraz and difenoconazole were (0.203 0±0.135 3)μg/mL and (0.675 9±0.394 2)μg/mL, respectively. The MICs of prochloraz and difenoconazole were 20 μg/mL and 40 μg/mL, respectively. The resistance frequencies of 375 tested strains to the two fungicides were both zero. The results provided a scientific basis for rational application of prochloraz and difenoconazole, and laid a foundation for the subsequent study on the resistance risk assessment. |
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