| 卢 晨1, 牛红莉1, 何小行1, 2, 尹军良1, 马东方1, 2*.小麦类病变LSD基因全基因组鉴定与转录组分析[J].植物保护,2021,47(6):180-189. |
| 小麦类病变LSD基因全基因组鉴定与转录组分析 |
| Genome-wide identification and transcriptome analysis of lesion simulating disease (LSD) gene family in wheat |
| 投稿时间：2020-07-29 修订日期：2020-09-10 |
| DOI：10.16688/j.zwbh.2020403 |
| 中文关键词: 类病变 小麦 同源性分析 蛋白特征 转录组分析 |
| 英文关键词:lesion simulating disease wheat homology analysis protein characteristics transcriptome analysis |
| 基金项目:湖北省教育厅科学研究计划重点项目（D20191305） |
| 作者 | 单位 | E-mail | | 卢 晨1, 牛红莉1, 何小行1, 2, 尹军良1, 马东方1, 2* | 1. 长江大学农学院, 湿地生态与农业利用教育部工程研究中心, 主要粮食作物产业化湖北省协同创新中心, 荆州 434025
2. 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193 | E-mail:madongfang1984@163.com |
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| 中文摘要: |
| 类病变(lesion simulating disease, LSD)基因编码锌指蛋白家族, 在细胞程序性死亡(PCD)和其他生物过程中发挥作用。本研究鉴定了普通小麦Triticum aestivum和其3种亚基因组供体乌拉尔图小麦T.urartu、野生二粒小麦T.dicoccoides和节节麦Aegilops tauschii中LSD基因家族。结果表明, 普通小麦、乌拉尔图小麦、野生二粒小麦和节节麦中分别含有24、4、10个和5个LSD基因; 同源性分析表明, 普通小麦相关的同源物共70对, 有38对旁系同源物(54%)。蛋白质特征显示, 13个(54%)TaLSD基因含有3个zf-LSD1结构域, 大部分TaLSD为碱性蛋白质、不稳定蛋白质和非亲水蛋白质; 转录组分析表明, 小麦LSD基因的表达量与禾谷镰刀菌、白粉菌和条锈菌的侵染紧密相连。 |
| 英文摘要: |
| The lesion simulating disease (LSD) genes encode a family of zinc finger proteins that play a role in programmed cell death (PCD) and other biological processes. In this study, we identified the LSD gene family in common wheat Triticum aestivum and three sub-genome donor species (T.urartu, T.dicoccoides and Aegilops tauschii). The results showed that the numbers of LSD genes were 24, 4, 10, and 5 in T.aestivum, T.urartu, T.dicoccoides and A.tauschii, respectively. Homology analysis showed that there were 70 pairs of homologues in wheat, and 38 (54%) pairs of paralogs. Protein characterization showed that there were 13 TaLSD genes (54%) containing three zf-LSD1 domains, most of which were basic proteins, unstable proteins and non-hydrophilic proteins. Transcriptome analysis showed that the expression level of wheat LSD genes was closely related to the infection by Fusarium graminearum, Blumeria graminis f.sp. tritici and Puccinia striiformis f.sp. tritici. |
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