| 黄洋1#, 关巍1#, 王铁霖2, 晏建红1, 田二渊1, 刘堰凤1, 刘博1, 杨玉文1, 赵廷昌1*.两类抗生素药剂对‘纽荷尔’脐橙黄龙病菌的抑制作用及根际细菌群落结构的影响[J].植物保护,2021,47(6):83-92. |
| 两类抗生素药剂对‘纽荷尔’脐橙黄龙病菌的抑制作用及根际细菌群落结构的影响 |
| Effects of two antibiotics on pathogen causing Huanglongbing of ‘newhall’ navel orange and the community structure of rhizosphere bacteria |
| 投稿时间：2020-04-27 修订日期：2020-06-19 |
| DOI：10.16688/j.zwbh.2020219 |
| 中文关键词: 柑橘黄龙病；根际细菌；宏基因组；抗生素 抑制作用 |
| 英文关键词:citrus Huanglongbing rhizosphere bacteria metagenome antibiotic inhibition effect |
| 基金项目:国家重点研发计划（2018YFD0201501）；中国农业科学院科技创新工程（CAAS-ASTIP）；中国农业科学院科技创新工程协同创新任务（CAAS-STCX2016013） |
| 作者 | 单位 | E-mail | | 黄洋1#, 关巍1#, 王铁霖2, 晏建红1, 田二渊1, 刘堰凤1, 刘博1, 杨玉文1, 赵廷昌1* | 1. 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京100193； 2. 道地药材国家重点实验室培育基地, 中国中医科学院中药资源中心, 北京100700 | E-mail:zhaotgcg@163.com |
|
| 摘要点击次数: 632 |
| 全文下载次数: 729 |
| 中文摘要: |
| 为探究磺胺二甲氧嘧啶钠盐（sulfadimethoxine sodium salt, SDM）和氨苄青霉素（ampicillin, AMP）对柑橘黄龙病（citrus Huanglongbing, HLB）的防效以及对柑橘根际细菌群落结构的影响, 本试验以‘纽荷尔’脐橙为研究对象, 采用0.5 g/L SDM和1 g/L AMP进行灌根, 使用TaqMan qPCR技术监测病树叶内黄龙病菌菌量的动态变化, 并利用宏基因组技术分析根际细菌群落结构的变化。结果显示, AMP连续处理后病树叶片内黄龙病菌菌含量在一定程度上受到抑制, 初步判断AMP对黄龙病有一定的抑制效果, SDM处理对黄龙病菌菌含量抑制效果较差；2类抗生素处理组中根际主要优势类群结构和相对丰度在门、属分类水平上均发生改变, 解磷细菌中慢生根瘤菌属Bradyrhizobium细菌的相对丰度在处理后有明显变化, pstA、pstC等部分磷循环功能基因相对丰度发生改变, 表明2类抗生素药剂均影响了‘纽荷尔’脐橙病树根际细菌群落结构。 |
| 英文摘要: |
| In order to investigate the control effects of sulfadimethoxine sodium salt (SDM) and ampicillin (AMP) on citrus Huanglongbing (HLB) and the effects of these two chemical agents on the citrus rhizosphere bacteria, the dynamic changes of the quantity of Candidatus Liberibacter asiaticus (CLas) in the leaves of ‘newhall’ navel orange was monitored by using TaqMan qPCR detection technology, and the changes of the bacterial community structure in rhizosphere soil was analyzed by using metagenomic technology. The results showed that the quantity of CLas in the leaves of diseased tree was lower than initial bacteria after root irrigation with 1 g/L AMP, indicating that AMP can suppressed content of the CLas in HLB-infected citrus plants, while the control effect was poor after root irrigation with 0.5 g/L SDM. The structure and relative abundance of the dominant rhizosphere bacteria were changed at the levels of phylum and genus. The relative abundance of Bradyrhizobium with phosphorus-dissolving ability and some phosphorous cycle-related functional genes such as pstA and pstC were changed after treatment with AMP and SDM. It suggested that both antibiotics affected the community structure of the rhizosphere bacteria in ‘newhall’ navel orange. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
