| 续海红1, 王燕飞2, 窦彦鑫1, 陈伟2*, 杨凯1, 3.山西苹果褪绿叶斑病毒的分布及遗传多样性分析[J].植物保护,2021,47(6):75-82. |
| 山西苹果褪绿叶斑病毒的分布及遗传多样性分析 |
| Analysis on distribution and genetic diversity of Apple leaf spot virus from Shanxi province |
| 投稿时间：2020-10-31 修订日期：2021-01-19 |
| DOI：10.16688/j.zwbh.2020578 |
| 中文关键词: 山西苹果 隐性病毒 苹果褪绿叶斑病毒 外壳蛋白 遗传变异 |
| 英文关键词:apple recessive viruses Apple chlorotic leaf spot virus coat protein genetic diversity |
| 基金项目:国家现代苹果产业技术体系晋中综合试验站(CARS-27); 山西省重点研发计划重点项目(201703D211002-5-3); 山西省科技成果转化引导专项(201904D131047) |
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| 中文摘要: |
| 苹果褪绿叶斑病毒Apple chlorotic leaf spot virus (ACLSV) 是侵染苹果的主要潜隐性病毒之一, 在我国苹果植株上发生普遍, 严重威胁我国苹果的品质与产量。本研究从山西省12个苹果主产区随机采集360份表现褪绿和斑驳等症状的苹果叶片作为研究样本, 通过RT-PCR检测, 360份样本中有209份样本为ACLSV阳性, 对209份阳性样本的外壳蛋白(coat protein, CP)基因进行分离、测序、克隆, 得到12个新的ACLSV分离物(分别命名为 Shanxi 1~Shanxi 12)。选择17个来自不同国家的分离物与12个新的ACLSV分离物在核苷酸和氨基酸层面上进行序列一致性和系统发育分析。结果显示, 29个ACLSV分离物被划分为2个不同进化群体。进一步对2个不同ACLSV群体进行选择压分析和中性检验, 结果表明, 组Ⅰ与组Ⅱ的ACLSV群体之间存在明显的遗传差异, 其中负向选择可能是ACLSV遗传变异的原因之一。本研究较全面地分析了ACLSV的发生、危害, 并对山西苹果的ACLSV分离物进行了遗传结构分析, 为山西苹果褪绿叶斑病毒病的防治提供了理论指导。 |
| 英文摘要: |
| Apple chlorotic leaf spot virus (ACLSV) is one of the main recessive viruses infecting apples. It occurs widely on apple plants in China, posing a serious threat to the quality and yield of apples in China. In this study, 360 samples of apple leaves with symptoms such as fading and mottling were randomly collected from 12 major apple production areas in Shanxi province. Based on RT-PCR test results, 209 out of 360 samples showed ACLSV-positive. The CP genes of 12 new ACLSV isolates, named as Shanxi 1—Shanxi 12, were isolated, sequenced and cloned from these positive samples. Phylogenetic analysis was performed based on the nucleotide and amino acid sequences of the CP genes of 29 ACLSV isolates (17 were obtained online). The results showed that these isolates were divided into two distinct evolutionary populations, with a possible relation to the region and host. The genetic diversity of different ACLSV populations was further studied. Selective pressure analysis and neutrality test showed that these isolates had significant genetic differences between group Ⅰ and group Ⅱ. Negative selection might play an important role in the genetic variation of ACLSV. The knowledge on the occurrence, damage, genetic structure and evolutionary mechanism of Shanxi ACLSV is useful for the design of sustainable management strategies for controlling ACLSV. |
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