| 陈晓1, 方靖怡1, 2, 王萌1, 沈晴3, 孙振军3*, 王备新1*.利用环境DNA-宏条形码技术监测苏州地区小管福寿螺的入侵[J].植物保护,2021,47(6):58-65. |
| 利用环境DNA-宏条形码技术监测苏州地区小管福寿螺的入侵 |
| Monitoring of the invasive species Pomacea canaliculata via environmental DNA metabarcoding in Suzhou city |
| 投稿时间：2020-07-24 修订日期：2020-08-25 |
| DOI：10.16688/j.zwbh.2020389 |
| 中文关键词: 环境DNA（eDNA） 高通量测序 小管福寿螺 生物入侵 |
| 英文关键词:environmental DNA high-throughput sequencing Pomacea canaliculata biological invasion |
| 基金项目:国家重点研发计划（2016YFC1201200） |
| 作者 | 单位 | E-mail | | 陈晓1, 方靖怡1, 2, 王萌1, 沈晴3, 孙振军3*, 王备新1* | 1. 南京农业大学植物保护学院, 南京210095；2. Wageningen University, Wageningen, The Netherlands 6700 AK；3. 苏州市植物保护植物检疫站, 苏州215008 | E-mail:孙振军15476536@qq.com；王备新wangbeixin@njau.edu.cn |
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| 中文摘要: |
| 小管福寿螺Pomacea canaliculata是我国南方重要的外来入侵有害生物, 目前已在江苏苏州的河道、湖塘等水体中广泛发生, 并呈现向北入侵趋势。及时准确地监测小管福寿螺在水体中的入侵扩散是有效防控其为害的关键。传统观察法受其生活史、发生状况及环境等影响, 难以在入侵早期及时监测。新兴的环境DNA-宏条形码(environmental DNA metabarcoding)技术可实现对入侵生物快速、灵敏的监测。本文分别采用环境DNA-宏条形码技术和传统观察方法对苏州地区河流、湖泊和运河共38个样点的小管福寿螺发生情况进行了检测。结果显示, 环境DNA-宏条形码技术检测到小管福寿螺的发生率（92.11%）远高于传统观察法（36.84%）。丰度阈值的设定和水体类型对环境DNA-宏条形码技术的检测结果有一定影响, 本研究为今后运用环境DNA-宏条形码技术进行福寿螺入侵监测提供技术支持。 |
| 英文摘要: |
| Pomacea canaliculata is a dangerous invasive species in southern China. At present, P.canaliculata has widely occurred in rivers, lakes, and other water bodies in Suzhou, Jiangsu province, and shows a trend of northward invasion. Timely and accurate monitoring of its invasion and spreading in water bodies is the key to effectively prevent and control its harm. Owing to the effect of its life history, occurrence, and environment, traditional observation cannot monitor P.canaliculata in the early stage of invasion. The emerging environmental DNA metabarcoding technology shows rapid and sensitive monitoring of invasive organisms. In this paper, environmental DNA metabarcoding and traditional observation methods were used to detect the occurrence of P.canaliculata in 38 samples of rivers, lakes, and canals in Suzhou. The results showed that the detection rate of P.canaliculata by environmental DNA metabarcoding technique (92.11%) was much higher than that by traditional observation method (36.84%). The setting of abundance threshold and water body type have a certain impact on the detection results by environmental DNA metabarcoding technology. This study would provide technical support for the future use of this technology to monitor Pomacea. |
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