| 李资聪1, 刘磊1, 杨斌2*, 严善春1*, 王桂荣2.黄野螟化学感受基因的鉴定与分析[J].植物保护,2021,47(6):34-48. |
| 黄野螟化学感受基因的鉴定与分析 |
| Identification and analysis of chemosensory genes in Heortia vitessoides |
| 投稿时间：2021-03-11 修订日期：2021-04-16 |
| DOI：10.16688/j.zwbh.2021151 |
| 中文关键词: 黄野螟 转录组 高通量测序 化学感受基因 |
| 英文关键词:Heortia vitessoides transcriptome high-throughput sequencing chemosensory genes |
| 基金项目:国家自然科学基金杰出青年基金（31725023）；深圳市科技计划（KQTD20180411143628272） |
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| 中文摘要: |
| 昆虫的化学感受系统在昆虫的取食、交配等多种行为中发挥着重要作用。黄野螟Heortia vitessoides是一种寡食性害虫, 幼虫具有聚集性、暴食性等特点, 对土沉香Aquilaria sinensis危害严重。本研究使用高通量测序技术对黄野螟成虫触角和口器的转录组进行了测序, 共鉴定到124个化学感受基因, 包括50个气味受体(odorant receptor, OR)基因, 19个离子型受体(ionotropic receptor, IR)基因, 17个味觉受体(gustatory receptor, GR)基因, 19个气味结合蛋白(odorant binding protein, OBP)基因, 17个化学感受蛋白(chemosensory protein, CSP)基因和2个感觉神经元膜蛋白(sensory neuron membrane protein, SNMP)基因。通过系统进化和差异表达分析, 对黄野螟化学感受基因的分化, 以及不同性别、不同组织间的表达差异进行系统性的研究。以气味受体为例, HvitOR42、HvitOR43和HvitOR48聚在传统的性信息素受体(pheromone receptor, PR)分支, 其中HvitOR42和HvitOR43在雄性成虫触角中特异性高表达, 这3个气味受体可能参与识别黄野螟雌性成虫释放的性信息素。此外, HvitOR20在雌性成虫触角中特异性高表达, 可能与雌性成虫交配和产卵等行为相关。本研究为进一步探究黄野螟的化学感受机制提供了分子基础, 为开发以化学生态学为基础的黄野螟绿色害虫防控技术提供了理论依据。 |
| 英文摘要: |
| Insect chemosensory system plays an important role in various behaviors such as feeding and mating. The yellow stem borer Heortia vitessoides is a kind of oligophagous pests against Aquilaria sinensis, causing serious damages by larval aggregation and overeating. In this study, high-throughput sequencing technology was used to obtain the transcriptomes in the antennae and mouthparts of H.vitessoides adults. As a result, 124 chemosensory genes were identified, including 50 odorant receptor (OR) genes, 19 ionotropic receptor (IR) genes, 17 gustatory receptor (GR) genes, 19 odorant binding protein (OBP) genes, 17 chemosensory protein (CSP) genes and two sensory neuron membrane protein (SNMP) genes. Phylogenetic and differential expression analyses were carried out to systematically study the gene differentiation and tissue specific expression of these chemosensory genes in H.vitessoides. Taking the odorant receptors as an example, HvitOR42, HvitOR43 and HvitOR48 clustered in the clade of traditional sex pheromone receptor, among which HvitOR42 and HvitOR43 were highly expressed in the male adult antennae, which were considered important in the recognition of sex pheromones produced by the females. In addition, HvitOR20 was highly expressed in the female antennae, which was considered to be involved in the female specific behaviors such as mating and oviposition. This study provided a molecular basis for further study of the chemosensory mechanism in H.vitessoides, and helps develop new methods for controlling H.vitessoides. |
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