| 刘建青1,2, 王永志2, 周 琦3, 黄淑敏3, 梁雨欣2,张春雨2, 魏 健1*, 程兆伟4*, 李小宇2*.吉林、黑龙江毛葱病毒病调查[J].植物保护,2021,47(5):271-274. |
| 吉林、黑龙江毛葱病毒病调查 |
| Investigation of virus disease on shallot in Jilin and Heilongjiang provinces |
| 投稿时间：2020-11-19 修订日期：2021-01-05 |
| DOI：10.16688/j.zwbh.2020617 |
| 中文关键词: 毛葱 病毒病 双重RT-PCR |
| 英文关键词:Allium cepa var. aggregatum virus disease duplex RT-PCR |
| 基金项目:农业农村部东北作物有害生物综合治理重点实验室开放基金（DB2018-09）;吉林省农业科技创新工程人才基金-研究生基金（C02100814） |
| 作者 | 单位 | E-mail | | 刘建青1,2, 王永志2, 周 琦3, 黄淑敏3, 梁雨欣2,张春雨2, 魏 健1*, 程兆伟4*, 李小宇2* | 1. 长春师范大学生命科学学院, 长春 130032
2. 吉林省农业科学院, 公主岭 136100
3. 吉林省靖宇县农业技术推广中心, 白山 135200
4. 吉林省农业技术推广总站, 长春 130033 | 魏健148050459@qq.com; 程兆伟25410763@qq.com; 李小宇lxyzsx@163.com |
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| 中文摘要: |
| 本研究为明确吉林和黑龙江省毛葱病毒病发生率,从两省5个地区共采集255份毛葱样品。根据毛葱4种主要病毒基因组序列设计特异性引物,对胡葱黄条病毒Shallot yellow stripe virus(SYSV)和青葱X病毒Shallot virus X (SVX)、洋葱黄矮病毒Onion yellow dwarf virus (OYDV)和葱潜隐病毒Shallot latent virus (SLV)进行双重RT-PCR检测。结果表明,229份样品检出病毒,带毒率为89.8%,SLV的检出率最高,达87.06%,OYDV次之,为36.86%,SYSV检出率偏低,为0.78%;同时存在病毒复合侵染,其中双病毒复合侵染为SLV和OYDV,检出率为33.3%;三病毒复合侵染为SYSV、OYDV和SLV,检出率为0.78%,未发现4种病毒复合侵染。本研究为吉林和黑龙江种植区毛葱病毒病防治提供了参考依据。 |
| 英文摘要: |
| To investigate the incidence of shallot virus diseases in Jilin and Heilongjiang provinces, 255 samples were collected. Four viruses specific primers were designed according to viral genomic sequences, Shallot yellow stripe virus (SYSV) and Shallot virus X (SVX), Onion yellow dwarf virus (OYDV) and Shallot latent virus (SLV) were detected by duplex RT-PCR. The results showed that 229 samples were positive, and the incidence of virus disease was 89.8%, of which 87.06% were infected with SLV, 36.86% with OYDV and 0.78% with SYSV. Coinfection of SLV and OYDV was the main type of multiple infections with the infection rate of 33.3%, and the mixed-infection of SYSV, OYDV and SLV was the main type of infection containing three kinds of viruses with an incidence of 0.78%. However, no four virus coinfection was detected. The results provide basis for the prevention and control of virus diseases in shallot planting areas of Jilin and Heilongjiang provinces. |
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