| 王泽平1, 宋修鹏1, 颜梅新1, 李毅杰1, 李 翔1,张小秋1, 唐红琴2*, 龙盛风3*.甘蔗NBS-LRR类抗梢腐病基因的筛选及定量表达分析[J].植物保护,2021,47(5):254-258. |
| 甘蔗NBS-LRR类抗梢腐病基因的筛选及定量表达分析 |
| Screening and quantitative analysis of NBS-LRR gene against pokkah boeng disease in sugarcane |
| 投稿时间：2020-06-10 修订日期：2020-06-30 |
| DOI：10.16688/j.zwbh.2020301 |
| 中文关键词: 甘蔗 梢腐病 转录组 NBS-LRR |
| 英文关键词:sugarcane pokkah boeng disease transcriptome NBS-LRR |
| 基金项目:国家自然科学基金青年科学基金(31801422); 中央引导地方科技发展专项(2021ZYZX2022); 广西农科院科技支撑(2015YM16);有机循环农业产业科技先锋队（桂家科盟202013） |
| 作者 | 单位 | E-mail | | 王泽平1, 宋修鹏1, 颜梅新1, 李毅杰1, 李 翔1,张小秋1, 唐红琴2*, 龙盛风3* | 1. 广西壮族自治区农业科学院甘蔗研究所，中国农业科学院甘蔗研究中心，农业农村部广西甘蔗生物技术与遗传改良重点实验室, 南宁 530007
2. 广西壮族自治区农业科学院农业资源与环境研究所, 南宁 530007
3. 广西壮族自治区农业科学院, 南宁 530007 | 唐红琴745784546@qq.com;龙盛风522377865@qq.com |
|
| 摘要点击次数: 426 |
| 全文下载次数: 377 |
| 中文摘要: |
| 本文旨在探讨核苷酸结合位点和富含亮氨酸重复(NBS-LRR)类基因参与甘蔗抗梢腐病菌侵染应答机制,为后续克隆关键抗病基因以及研究抗病机理提供理论依据。试验利用Illumina高通量转录组测序技术检测高抗梢腐病品种‘粤糖94-128’和高感梢腐病品种‘桂糖37号’接种前后NBS-LRR类抗梢腐病基因的表达情况,然后设计引物对显著差异表达基因进行不同接种时期下的荧光定量PCR验证。结果表明,16个NBS-LRR类基因在甘蔗叶片受到梢腐病菌侵染后持续上调表达,但表达趋势存在两种情况,13个基因在诱导后7 d显著或极显著上调表达,3个基因在诱导7 d后上调表达不显著,在诱导14 d后才显著上调表达。据此可将其分为瞬时基础防御基因(0~7 d)和滞后特异性防御基因(14~21 d),确定NBS-LRR类基因参与甘蔗防御梢腐病菌的侵染。 |
| 英文摘要: |
| The molecular mechanism of nucleotide binding sites and leucine-rich replication (NBS-LRR) genes resistant to pokkah boeng disease (PBD) in sugarcane was explored to provide a theoretical basis for cloning resistant genes. This study firstly used Illumina high-throughput transcriptome sequencing technology to detect NBS-LRR genes against PBD with typical resistant and susceptive sugarcane materials, and then designed primers to verify the expression characteristics of significant differentially expressed NBS-LRR genes under different inoculation periods using real-time quantitative PCR (qRT-PCR). The results showed that 16 NBS-LRR-like genes steadily increased expression when sugarcane leaves were infected with the PBD pathogen, including two trends in expression: 13 genes significantly or extremely significantly increased expression at 7 d post-inoculation, but three genes did not significantly increased expression at 7 d post-inoculation, but significantly increased expression at 14 d post-inoculation. Therefore, these genes could be divided into instantaneous basic defense genes (0 d to 7 d) and specific lagging defense genes (14 d to 21 d), suggesting that NBS-LRR genes are actively involved in resistance against the PBD pathogen. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
