| 赵倩楠1, 黄明娇2, 危学高2, 杨 静2, 杜田华2, 殷 城2,向文胜1, 杨 鑫2, 张友军1,2*.烟粉虱细胞色素CYP6EM1基因的克隆及对吡虫啉抗性的作用[J].植物保护,2021,47(5):171-177. |
| 烟粉虱细胞色素CYP6EM1基因的克隆及对吡虫啉抗性的作用 |
| Cloning of cytochrome P450 gene CYP6EM1 and its function in imidacloprid resistance in whitefly, Bemisia tabaci |
| 投稿时间：2020-06-02 修订日期：2020-07-16 |
| DOI：10.16688/j.zwbh.2020279 |
| 中文关键词: 烟粉虱 吡虫啉 细胞色素P450 RNA干扰 |
| 英文关键词:Bemisia tabaci imidacloprid cytochrome P450 RNA interference |
| 基金项目:国家重点研发计划（2019YFD1002100）;现代农业产业技术体系（CARS-24-C-02）;蔬菜有害生物控制与优质栽培北京市重点实验室和中国农业科学院科技创新工程 (CAAS-ASTIP-IVFCAAS) |
| 作者 | 单位 | E-mail | | 赵倩楠1, 黄明娇2, 危学高2, 杨 静2, 杜田华2, 殷 城2,向文胜1, 杨 鑫2, 张友军1,2* | 1. 东北农业大学生命科学学院, 哈尔滨 150030
2. 中国农业科学院蔬菜花卉研究所, 北京 100081 | zhangyoujun@caas.cn |
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| 中文摘要: |
| 烟粉虱是一种世界性农业害虫,其防治手段以化学防治为主,新烟碱类杀虫剂吡虫啉常年用于防治烟粉虱,田间烟粉虱已经形成严重的抗药性。本研究通过分析烟粉虱吡虫啉抗性和敏感种群,发现细胞色素CYP6EM1基因在吡虫啉抗性品系中上调了4.7倍,进而克隆了其全长基因,进行了荧光定量PCR分析,发现该基因在吡虫啉抗性烟粉虱3龄若虫期和雄虫成虫期过量表达,并且在抗性成虫胸部和腹部过量表达。最后通过RNA干扰的方法使成虫的CYP6EM1基因表达量下降了54.8%,之后发现当烟粉虱暴露于吡虫啉时死亡率显著升高了39.65%,这表明CYP6EM1与烟粉虱对吡虫啉抗性的形成相关。研究结果对于揭示烟粉虱对吡虫啉产生抗性的机制有帮助,也为烟粉虱抗性水平田间监测及烟粉虱综合治理提供理论依据。 |
| 英文摘要: |
| Whitefly, Bemisia tabaci, is a global harmful pest, and insecticides were widely used to control this pest. Imidacloprid, one of the neonicotinoid insecticides, has been used to control whitefly for many years. However, resistance to imidacloprid has been developed in field strains. In this study, the imidacloprid resistant and susceptible populations were compared, and a cytochrome P450 gene, CYP6EM1 was found to be over-expressed in resistant strain for 4.7 fold. The full length of CYP6EM1 was cloned and the mRNA expression level was analyzed by qRT-PCR. The results showed that CYP6EM1 was over-expressed in the third instar nymph and male adult of B.tabaci of imidacloprid resistance, and abound in thorax and abdomen of resistant adult. Moreover, the expression level of CYP6EM1 gene in adults was reduced by 54.8% by RNA interference. Then, it was found that the mortality of B.tabaci significantly increased by 39.65% when exposed to imidacloprid. This study is conducive to revealing the imidacloprid resistance mechanism of B.tabaci and provides a theoretical significance to determine the status of imidacloprid resistance in B.tabaci in the field and further establish the IPM strategies for whiteflies. |
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