| 苏 琴1#, 崔丽贤1#, 陈起民2, 谢慧婷1, 陈锦清1,秦碧霞1, 朱英芝2, 李战彪1, 蔡健和1*.侵染广西番茄的番茄黄化曲叶病毒的分子鉴定[J].植物保护,2021,47(5):146-151. |
| 侵染广西番茄的番茄黄化曲叶病毒的分子鉴定 |
| Molecular identification of Tomato yellow leaf curl virus in Guangxi province |
| 投稿时间：2020-06-01 修订日期：2020-07-15 |
| DOI：10.16688/j.zwbh.2020278 |
| 中文关键词: 番茄 番茄黄化曲叶病毒(TYLCV) 分子鉴定 序列分析 |
| 英文关键词:tomato Tomato yellow leaf curl virus (TYLCV) molecular identification sequence analysis |
| 基金项目:广西创新驱动发展专项(桂科AA17204041);广西农业科学院基本科研业务费专项(2015YT42) |
| 作者 | 单位 | E-mail | | 苏 琴1#, 崔丽贤1#, 陈起民2, 谢慧婷1, 陈锦清1,秦碧霞1, 朱英芝2, 李战彪1, 蔡健和1* | 1. 广西农业科学院植物保护研究所, 广西作物病虫害生物学重点实验室, 南宁 530007
2. 广西民族大学海洋与生物技术学院, 南宁 530006 | caijianhe@gxaas.net |
|
| 摘要点击次数: 479 |
| 全文下载次数: 279 |
| 中文摘要: |
| 番茄黄化曲叶病毒病是番茄生产上的毁灭性病害,严重影响番茄产量及品质。2019年从广西百色市采集疑似番茄黄化曲叶病叶片样品,采用滚环扩增(RCA)及基因克隆等方法,获得了4个烟粉虱传双生病毒的全基因序列,4个分离物的全长序列分别为2 776、2 781、2 752、2 781 bp,均编码6个开放阅读框;核苷酸相似性比较发现,4个分离物彼此间的相似性均在90%以上,与已报道的番茄黄化曲叶病毒Tomato yellow leaf curl virus (TYLCV)各分离物间的相似性也在91%以上;系统进化分析表明,TYLCV广西分离物BS-2和BS-4与TYLCV-Hunan、BS-1和BS-3与TYLCV-YN6553等分离物处于独立的小分支,说明TYLCV广西分离物与TYLCV-Hunan、TYLCV-YN6553具有较近的亲缘关系。本研究首次报道TYLCV在广西发生。 |
| 英文摘要: |
| Tomato yellow leaf curl disease (TYLCD) is one of the devastating diseases in tomato production, seriously reducing the yield and quality of tomato. In 2019, suspected TYLCD leaf samples were collected from Baise city, Guangxi Zhuang autonomous region. Four complete gene sequences of begomovirues transmitted by Bemisia tabaci were obtained by using rolling circle amplification (RCA) and gene cloning. The full-length sequences of the four isolates were 2 776, 2 781, 2 752 bp, and 2 781 bp, respectively, encoding six open reading frames. The nucleotide sequence identities between the four isolates were over 91%, and their identities against other Tomato yellow leaf curl virus (TYLCV) isolates were over 91%. Phylogenetic analysis showed that TYLCV Guangxi isolates BS-2 and BS-4 clustered with TYLCV-Hunan, and BS-1 and BS-3 clustered with TYLCV-YN6553 in a sub-clade, indicating their close relation. This is the first report of TYLCV infecting tomato in Guangxi. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
