| 吴 迟 2, 何明远 2, 3, 欧阳小庆 2, 孙 田 2, 王长宾 2, 才 冰 2, 孟学林 1*.超高效液相色谱-串联质谱检测小麦中 唑啉草酯及其代谢物M4[J].植物保护,2021,47(4):148-154. |
| 超高效液相色谱-串联质谱检测小麦中 唑啉草酯及其代谢物M4 |
| Determination of pinoxaden and its metabolite M4 in wheat by UPLC-MS/MS |
| 投稿时间：2020-11-11 修订日期：2020-12-17 |
| DOI：10.16688/j.zwbh.2020599 |
| 中文关键词: 唑啉草酯 唑啉草酯代谢物M4 超高效液相色谱-串联质谱 小麦 |
| 英文关键词:pinoxaden pinoxaden metabolite M4 UPLC-MS/MS wheat |
| 基金项目:无基金 |
| 作者 | 单位 | E-mail | | 吴 迟 2, 何明远 2, 3, 欧阳小庆 2, 孙 田 2, 王长宾 2, 才 冰 2, 孟学林 1* | 1. 广西工业职业技术学院, 南宁 530001
2. 北京依科世福科技有限公司, 北京 101102
3. 广西思浦林科技有限公司, 南宁 530001 | mxl992@126.com |
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| 中文摘要: |
| 本研究采用QuEChERS方法进行样品前处理, 优化提取溶剂及净化剂, 建立了超高效液相色谱-串联质谱仪(UPLC-MS/MS)对小麦麦粒和秸秆基质中唑啉草酯及其代谢物M4的检测方法, 研究表明, 在0.000 5~0.5 mg/L范围内, 唑啉草酯及其代谢物M4的浓度与色谱峰面积均呈现良好的线性关系?唑啉草酯在麦粒和秸秆中的平均回收率分别为77.6%~90.4%?76.7%~84.4%, 唑啉草酯代谢物M4在麦粒和秸秆中的平均回收率分别为81.9%~89.4%和74.5%~89.2%?唑啉草酯在麦粒?麦秆中的定量限分别为0.001 mg/kg?0.01 mg/kg; 唑啉草酯代谢物M4在麦粒?麦秆中的定量限分别为0.01 mg/kg?0.05 mg/kg?本研究为准确?高效?经济地检测小麦中唑啉草酯及唑啉草酯代谢物M4提供了可靠依据? |
| 英文摘要: |
| In this study, the QuEChERS method was used for sample pretreatment, and the extraction solvent and purification agent were optimized. An ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was established for the determination of pinoxaden and its metabolite M4 in wheat grains and straw substrate. In the range of 0.000 5-0.5 mg/L, there was a good linear relationship between the concentration and the peak area. The average recoveries of pinoxaden in wheat and straw were 77.6%-90.4% and 76.7%-84.4%, respectively, and the average recoveries of M4 in wheat and straw were 81.9%-89.4% and 74.5%-89.2%, respectively. The limit of quantitation (LOQ) of pinoxaden in wheat grains and straws were 0.001 mg/kg and 0.01 mg/kg, respectively, while those of pinoxaden metabolite M4 were 0.01 mg/kg and 0.05 mg/kg, respectively. This study provided a reliable basis for the accurate, efficient and economical detection of pinoxaden and its metabolite M4 in wheat. |
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