| 朱 娜, 张树武*, 徐秉良*, 刘 佳.解淀粉芽胞杆菌TS-1203对苹果炭疽叶枯病菌的抗生作用[J].植物保护,2021,47(4):46-51. |
| 解淀粉芽胞杆菌TS-1203对苹果炭疽叶枯病菌的抗生作用 |
| Inhibitory effects of Bacillus amyloliquefaciens TS-1203 on Glomerella cingulata |
| 投稿时间：2020-04-06 修订日期：2020-05-21 |
| DOI：10.16688/j.zwbh.2020174 |
| 中文关键词: 苹果炭疽叶枯病菌 解淀粉芽胞杆菌 菌落生长 孢子萌发 抑制作用 |
| 英文关键词:Glomerella cingulata Bacillus amyloliquefaciens colony growth spore germination inhibitory effect |
| 基金项目:国家重点研发计划(2016YFD0201100); 甘肃农业大学人才专项经费(2017RCZX-07); 甘肃农业大学国家重点实验室开放基金(GSCS-2017-1) |
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| 中文摘要: |
| 为明确解淀粉芽胞杆菌TS-1203对苹果炭疽叶枯病菌的抗生作用, 采用平板对峙法和菌丝生长速率法以及孢子萌发法测定了解淀粉芽胞杆菌TS-1203对苹果炭疽叶枯病菌菌落生长和孢子萌发的抑制作用?结果表明:菌株TS-1203对苹果炭疽叶枯病菌具有显著的抑制作用, 其菌液对病菌的抑制率高达70.4%; 其发酵液对苹果炭疽叶枯病菌的抑制率随着发酵液浓度的增加而升高, 尤其当发酵液浓度为15%时, 其抑菌率为 59.0%; 发酵液处理苹果炭疽叶枯病菌孢子24 h后, 其萌发抑制率与芽管生长抑制率分别为78.2%与64.3%, 且芽管出现畸形; 利用TS-1203发酵液处理苹果离体叶片后接种炭疽叶枯病菌, 其防效为70.1%?综合分析表明, 解淀粉芽胞杆菌TS-1203对苹果炭疽叶枯病菌具有良好的抑制作用? |
| 英文摘要: |
| The inhibitory effect of Bacillus amyloliquefaciens TS-1203 on the mycelial growth and spore germination of Glomerella cingulata were studied by using confrontation method, mycelial growth rate method and spore germination method. The results showed that the strain TS-1203 had a significant inhibitory effect on G.cingulata. The inhibitory rate was as high as 70.4%. The inhibitory rate of its fermentation broth on G.cingulata increased with increasing fermentation broth concentration. At the concentration of 15%, the inhibition rate was 59.0%. After treatment with the fermentation broth for 24 h, the inhibitory rates of germination and germ tube growth were 78.2% and 64.3%, respectively, with deformed germ tubes. The application of TS-1203 fermentation broth treated leaves before inoculation with G. cingulata in vitro, showed the control effect of 70.1%. Our results indicate that B.amyloliquefaciens TS-1203 had significant inhibitory effect on G.cingulata. |
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