| 孙丹丹, 全玉东, 王月琴, 王振营, 何康来*.转Bt基因玉米(瑞丰125、DBN9936、DBN9978)对亚洲玉米螟的抗虫效果研究[J].植物保护,2021,47(3):206-211. |
| 转Bt基因玉米(瑞丰125、DBN9936、DBN9978)对亚洲玉米螟的抗虫效果研究 |
| Resistance of transgenic Bt maize (Ruifeng 125, DBN9936 & DBN9978) to Asian corn borer |
| 投稿时间：2020-01-03 修订日期：2020-03-13 |
| DOI：DOI:10.16688/j.zwbh.2020008 |
| 中文关键词: 转基因玉米 Bt杀虫蛋白 亚洲玉米螟 寄主抗性 |
| 英文关键词:transgenic maize Bt insecticidal protein Ostrinia furnacalis host plant resistance |
| 基金项目:转基因生物新品种培育重大专项(2016ZX08003-001) |
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| 中文摘要: |
| 评价转Bt基因玉米对靶标生物亚洲玉米螟的杀虫作用是转基因玉米研发的重要一环。本文采用室内生测法对3种转Bt基因抗虫玉米‘瑞丰125’(表达Cry1Ab/Cry2Aj杀虫蛋白),‘DBN9936’‘DBN9978’(表达Cry1Ab杀虫蛋白)对亚洲玉米螟敏感品系ACB-S及抗Cry1Ab品系ACB-AbR、抗Cry1Ac品系ACB-AcR、抗Cry1F品系ACB-FR、抗Cry1Ah品系ACB-AhR、抗Cry1Ie品系ACB-IeR的杀虫活性进行测定,同时采用心叶期和抽丝期人工接虫法进行田间抗虫效果鉴定。结果表明,取食3种Bt玉米的ACB-S幼虫, 3 d死亡率100%,而取食对照常规玉米3 d存活率100%。取食3种Bt玉米的5个抗性品系幼虫除ACB-AbR和ACB-AcR有2%~6%的个体存活4~5 d, 6 d死亡率也达到了100%,其余品系均在3 d全部死亡,而取食对照玉米5~6 d的死亡率仅为4%~14%,差异显著。田间心叶期食叶级别及穗期活虫数、雌穗被害和茎秆被蛀等为害等级说明3种Bt玉米高抗亚洲玉米螟。明确了‘瑞丰125’‘DBN9936’和‘DBN9978’对亚洲玉米螟有很高的杀虫活性和田间防治效果。5个Bt蛋白抗性亚洲玉米螟品系幼虫在常规玉米上显示一定的适合度劣势。 |
| 英文摘要: |
| Evaluation for resistance to the targets such as Asian corn borer (ACB), Ostrinia furnacalis, is an important step of research and development novel insect resistant transgenic Bt maize. In present research, three kinds of insect resistant transgenic Bt maize, i.e. ‘Ruifeng 125’ expressing Cry1Ab/Cry2Aj protein, ‘DBN9936’ and ‘DBN9978’ expressing Cry1Ab protein, were evaluated in the laboratory and field. Laboratory bioassays were conducted by exposing neonates of ACB susceptible strain (ACB-S), Cry1Ab resistant strain (ACB-AbR), Cry1Ac resistant strain (ACB-AcR), Cry1F resistant strain (ACB-FR), Cry1Ah resistant strain (ACB-AhR), and Cry1Ie resistant strain (ACB-IeR) to fresh whorl leaves, respectively. Field trials were conducted by artificial infestation of ACB at whorl and silking stages. Mortalities were 100% within 3 days when ACB-S larvae fed on three Bt maize leaf tissues, whereas all larvae survived when they fed on the control of conventional maize leaf tissues. When ACB-AbR and ACB-AcR larvae fed on three Bt maize leaf tissues, 2%-6% of larvae could survival for 4-5 days, but not longer than six days. In contrast, there were 4%-14% of larval mortalities when these larvae fed on control within six days. Leaf feeding ratings from whorl stage infestation, larval survivals and ear and stalk bored and tunnels indicated that three Bt maize varieties were highly resistant to ACB. In conclusion, Bt maize ‘Ruifeng 125’ ‘DBN9936’ and ‘DBN9978’ are highly toxic to ACB and could provide season-long protection against ACB. Laboratory selected Cry1Ab, Cry1Ac, Cry1Ah, Cry1F, and Cry1Ie resistant strains demonstrate certain fitness cost. |
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