徐 哲1,2, 刘太国1,2,3*, 刘 博1, 高 利1, 陈万权1,3*.我国四大麦区小麦籽粒镰刀菌毒素检测与污染分析[J].植物保护,2021,47(3):200-205. |
我国四大麦区小麦籽粒镰刀菌毒素检测与污染分析 |
Determination and contamination analysis of Fusarium mycotoxins in wheat grains from four different wheat regions |
投稿时间:2020-01-17 修订日期:2020-03-04 |
DOI:DOI:10.16688/j.zwbh.2020045 |
中文关键词: 超高效液相色谱-串联质谱 镰刀菌毒素 小麦 |
英文关键词:UPLC-MS/MS Fusarium mycotoxin wheat |
基金项目:国家重点研发计划(2016YFD0300700,2018YFD0200500);中央级公益性科研院所基本科研业务费(Y2017XM01,S2020XM02);国家现代农业产业技术体系(CARS-3);中国农业科学院科技创新工程(CAAS-ASTIP) |
作者 | 单位 | E-mail | 徐 哲1,2, 刘太国1,2,3*, 刘 博1, 高 利1, 陈万权1,3* | 1. 植物病虫害生物学国家重点实验室, 中国农业科学院植物保护研究所, 北京 100193 2. 农业农村部农产品质量安全生物性危害因子(植物源)控制重点实验室, 北京 100193 3. 农业农村部国家植物保护甘谷观测实验站, 天水 741200 | 刘太国tgliu@ippcaas.cn; 陈万权wqchen@ippcaas.cn |
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中文摘要: |
为明确自然发病条件下我国小麦主产区小麦籽粒镰刀菌毒素污染情况,用超高效液相色谱-串联质谱仪(UPLC-MS/MS)测定了长江中下游冬麦组(CZX)、黄淮冬麦区南片冬水组(HNS)、黄淮冬麦区北片水地组(HBS)、北部冬麦区水地组(BBS)180份小麦样品中DON、3ADON、15ADON、T-2、HT-2和ZEN共6种镰刀菌毒素含量并对其进行比较分析。结果显示:来自CZX小麦籽粒中的DON、3ADON与ZEN毒素含量显著高于其他麦区;T-2毒素在CZX小麦籽粒中含量显著低于其他麦区;15ADON毒素含量在HNS与CZX、HBS与BBS以及CZX与BBS差异不显著,其他麦区间该毒素含量差异显著,15ADON在HNS含量最高;检出率方面,HT-2毒素均未检出;ZEN、3ADON检出率较低,DON、15ADON和T-2毒素的检出率较高。 |
英文摘要: |
To clarify the contamination level of Fusarium mycotoxins in major wheat-producing zones under natural infected condition in China, ultra performance liquid chromatography-tandem mass spectrometery was used to detect six kinds of mycotoxins including DON,3ADON,15ADON,T-2,HT-2 and ZEN in 180 wheat cultivars, which come from the winter wheat of the middle and lower reaches of Yangtze River (CZX), the irrigated winter wheat in south of Huang-Huai region (HNS), the irrigated winter wheat in north of Huang-Huai region (HBS), the irrigated winter wheat in north of China(BBS). The results showed the contamination of DON, 3ADON and ZEN toxins in CZX was significantly higher than that in other regions, and the contamination of T-2 toxin from CZX was lower than that in other regions. There was no significant difference in the contamination of 15ADON toxin between HNS and CZX, HBS and BBS, CZX and BBS, while there was significant difference between the other wheat regions. The contamination of 15ADON toxin was highest in HSN. HT-2 toxin was not detected. The detection rate of ZEN and 3ADON was relatively low, while that of DON, 15ADON and T-2 toxin was high. |
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