| 叶乃玮, 王承芳, 干华磊, 吴紫燕, 糜芳, 毛伟力*.多黏类芽胞杆菌Paenibacillus polymyxa菌株P1防治黄瓜霜霉病的研究[J].植物保护,2021,47(2):271-275. |
| 多黏类芽胞杆菌Paenibacillus polymyxa菌株P1防治黄瓜霜霉病的研究 |
| Control effect of Paenibacillus polymyxa strain P1 against cucumber downy mildew |
| 投稿时间：2019-12-13 修订日期：2020-02-27 |
| DOI：10.16688/j.zwbh.2019689 |
| 中文关键词: 黄瓜霜霉病 室内药效 田间药效测试 多黏类芽胞杆菌 生防菌 |
| 英文关键词:cucumber downy mildew indoor test field efficacy test Paenibacillus polymyxa biocontrol agent |
| 基金项目:大理州毛伟力专家工作站（大科联发［2019］号） |
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| 中文摘要: |
| 通过离体叶片筛选法, 得到1株对黄瓜霜霉病原菌有较强抑制作用的多黏类芽胞杆菌Paenibacillus polymyxa菌株P1。通过液体发酵和剂型处理, 将 P1制成含量2×109 cfu/mL的悬浮剂分别进行室内和大田药效测试。室内药效试验结果表明, P1-SC悬浮剂对黄瓜霜霉病的防治效果与用药浓度呈正相关。当使用浓度为1.25×107 cfu/mL 时, P1悬浮剂对霜霉病的防治效果与化学农药250 g/L嘧菌酯SC的相似; 当使用浓度为5.0×107 cfu/mL时, 防治效果显著大于嘧菌酯(P≤0.05)。 2017年和2018年连续两年大田药效测试结果显示, 经P1-SC处理(3 000 mL/hm2)的黄瓜植株霜霉病病情指数(2.53, 2.48)显著低于经250 g/L嘧菌酯SC(600 mL/hm2)处理的植株(4.33、6.22), 防治效果(86.16%, 89.90%)显著高于后者(76.34%、74.95%)(P≤0.05)。 |
| 英文摘要: |
| By in vitro detached leaf assay, a strain P1 of Paenibacillus polymyxa was obtained, with strong antagonistic ability against the pathogen of cucumber downy mildew. Through the processes of liquid fermentation and formulation, P1 was prepared into a suspension concentrate (SC) containing 2×109 cfu/mL, which was used for indoor and field efficacy tests. Indoor test showed that it was positively correlated with the application concentration of P1-SC on the efficacy of controlling cucumber downy mildew. When the application concentration was at 1.25×107 cfu/mL, the control efficacy was equal to that of azoxystrobin 250 g/L SC. When the concentration was at 5.0×107 cfu/mL, the efficacy was significantly greater than (P≤0.05) that of azoxystrobin 250 g/L SC. Field tests in 2017 and 2018 showed that when treated with P1-SC (3 000 mL/hm2), the disease index (2.53, 2.48) was significantly lower than that of azoxystrobin 250 g/L SC(600 mL/hm2), while the control efficacy (86.16%, 89.90%)was significantly higher. |
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