| 温欣1, 秦红艳1, 艾军1, 2*, 王月1, 韩先焱1, 李昌禹1.软枣猕猴桃种质资源溃疡病抗性鉴定方法的建立与评价[J].植物保护,2021,47(2):193-199. |
| 软枣猕猴桃种质资源溃疡病抗性鉴定方法的建立与评价 |
| Establishment and evaluation of resistance identification method for Pseudomonas syringae pv. actinidiae disease in Actinidia arguta germplasm resources |
| 投稿时间：2019-12-27 修订日期：2020-02-24 |
| DOI：10.16688/j.zwbh.2019722 |
| 中文关键词: 软枣猕猴桃 抗性评价 细菌性溃疡病 |
| 英文关键词:Actinidia arguta disease resistance evaluation Pseudomonas syringae pv.actinidiae disease |
| 基金项目:中央级公益性科研院所基本科研业务费专项(1610342020011); 国家自然科学基金(31870673); 吉林省科技发展计划(20170203006NY) |
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| 中文摘要: |
| 以51份软枣猕猴桃Actinidia arguta种质资源为材料, 以中华猕猴桃Actinidia chinensis Planch ‘红阳’、美味猕猴桃Actinidia chinensis var. deliciosa‘徐香’为对照, 利用丁香假单胞菌猕猴桃致病变种Pseudomonas syringae pv.actinidiae PSAM228对软枣猕猴桃离体枝条和叶片进行人工接种和溃疡病抗性评价。结果表明, 不同年份半木质化离体枝条接种后的感病结果基本一致并达极显著相关水平, 相关系数为0.935 9, 半木质化离体枝条接种可作为软枣猕猴桃溃疡病抗性鉴定的方法; 根据病情指数将软枣猕猴桃种质资源按感病程度分为高抗、中抗、感病、中感和高感5个级别, 其中高抗资源33份, 中抗资源18份, 没有感病、中感和高感级次的种质资源。 |
| 英文摘要: |
| With 51 Actinidia arguta resources as tested materials, and Actinidia chinensis Planch ‘Hongyang’ and Actinidia chinensis var. deliciosa‘Xuxiang’ as control, the resistance of the resources to canker disease was evaluated by inoculating Pseudomonas syringae pv.actinidiae PSAM228 on the branches and leaves of Actinidia arguta in vitro. The results showed that the infection in the inoculated branches were nearly the same in two years, which reached a very significant correlation level, with the correlation coefficient of 0.935 9. Therefore, inoculation of the in vitro branches could be used for identification and evaluation of the resistance to canker disease in A.arguta. According to the disease index, the level of the disease resistance was divided into five levels, that is highly resistant, moderately resistant, susceptible, moderately susceptible and highly susceptible. There are 33 highly resistant resources and 18 moderately resistant resources, while no susceptible, moderately susceptible and highly susceptible resources. |
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