| 刘梅, 王彩霞, 燕继晔, 贾静怡, 李兴红*.辽宁省北镇市葡萄叶斑病的病原鉴定[J].植物保护,2021,47(2):185-188. |
| 辽宁省北镇市葡萄叶斑病的病原鉴定 |
| Identification of the pathogen causing leaf spot on grape in Beizhen city of Liaoning province |
| 投稿时间：2019-11-19 修订日期：2020-02-01 |
| DOI：10.16688/j.zwbh.2019631 |
| 中文关键词: 葡萄 叶斑病 多基因系统发育 平头炭疽菌 |
| 英文关键词:grape leaf spot multi-gene phylogeny Colletotrichum truncatum |
| 基金项目:国家葡萄产业技术体系（CARS-29-bc-2）；北京市科技计划项目（D171100001617002） |
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| 中文摘要: |
| 近年来辽宁省北镇市出现一种葡萄叶斑病, 症状表现为圆形或椭圆形小黑斑, 病斑周围褪绿变黄, 为明确引起该病的病原菌, 利用组织分离法对病样进行分离纯化, 采用多基因系统发育学分析(ITS、GAPDH、CHS-1、ACT、TUB2基因)和形态学特征观察对纯化后的菌株进行鉴定, 并结合柯赫氏法则对其进行验证。结果确定病原菌为平头炭疽菌Colletotrichum truncatum. |
| 英文摘要: |
| In recent years, a grape leaf spot disease appeared in Beizhen city of Liaoning province. The symptoms included small black round or oval spots, around the spots leaf faded green and turned yellow. Based on tissue isolation method, multi-gene phylogenetic analysis (ITS, GAPDH, CHS-1, ACT, TUB2), morphological characters and Koch’s postulates, the pathogen was identified as Colletotrichum truncatum. |
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