| 纪晓彬1, 3, 王 丹2, 刘 政3, 李 冉2, 宋 健2, 张丹丹2, 陈捷胤2, 戴小枫2, 林克剑1, 2, 3*.芽胞杆菌BvR001对大丽轮枝菌抑制效果研究[J].植物保护,2021,47(1):40-47. |
| 芽胞杆菌BvR001对大丽轮枝菌抑制效果研究 |
| Inhibitory efficacy of BvR001 against Verticillium dahliae |
| 投稿时间：2019-10-22 修订日期：2020-02-09 |
| DOI：DOI:10.16688/j.zwbh.2019568 |
| 中文关键词: 芽胞杆菌 可湿性粉剂 黄萎病 生物防治 |
| 英文关键词:Bacillus spp. wettable powder Verticillium wilt biological control |
| 基金项目:国家重点研发计划(2017YFD0201904, 2017YFD0200601); 国家自然科学基金(31671986, 31970142); 中国农业科学院基本科研业务费专项(Y2018PT70) |
| 作者 | 单位 | E-mail | | 纪晓彬1, 3, 王 丹2, 刘 政3, 李 冉2, 宋 健2, 张丹丹2, 陈捷胤2, 戴小枫2, 林克剑1, 2, 3* | 1. 石河子大学农学院, 新疆绿洲农业病虫害治理与植保资源利用重点实验室, 石河子 832003
2. 中国农业科学院植物保护研究所, 北京 100193
3. 新疆农垦科学院植物保护研究所, 石河子 832000 | E-mail:linkejian@caas.cn |
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| 中文摘要: |
| 为研发绿色?安全?高效的作物黄萎病生物防治产品, 本研究以生防菌株BvR001为研究对象, 通过对黄萎病菌的抑菌效果测定?可湿性粉剂研制?在寄主根际的定殖能力与防效测定, 明确该菌株对黄萎病具有防控效果?gyrB序列检测和系统发育树构建分析表明, 该菌株属于芽胞杆菌属, 并且与贝莱斯芽胞杆菌Bacillus velezensis进化关系最近, 推测其可能是贝莱斯芽胞杆菌; 平板对峙试验表明, 该菌对2种基因型(落叶型和非落叶型)大丽轮枝菌Vd991和D08047菌落扩展的抑制率分别为78.6%和85%; 研发了该菌的可湿性粉剂配方(WPBvR001):吸附量为1.2 L/kg的发酵液-硅藻土母粉87%?十二烷基磺酸钠5%?木质素磺酸钠5%?羧甲基纤维素钠2%?维生素C 1%, 该配方得到的制剂活芽胞量为7.65×108 cfu/g?润湿时间38 s?悬浮率为62.16%?杂菌率为0?pH 6.89±0.02?细度通过率(≤45 μm)99.99%?干燥减量0.67%; 利用烟草和棉花测试发现, 该菌剂对寄主植物安全无毒, 且能有效定殖于寄主根际, 显著降低了黄萎病菌繁殖扩展, 对黄萎病具有良好的防效? |
| 英文摘要: |
| To develop safe and effective biological control measures for Verticillium wilt, a series of studies on strain BvR001 were conducted, including its taxonomic characterization, colonization of tobacco rhizosphere, its efficacy for controlling Verticillium wilt and development of a wettable powder (WPBvR001) formulation to facilitate field application of the product. These studies confirmed that BvR001 could control Verticillium wilt on host plants such as cotton and tobacco. Gyrase B subunit gene gyrB sequence and the phylogenetic analyses showed that this strain was most closely related to Bacillus velezensis. Co-inoculation of BvR001 and two strains of Verticillium dahliae (defoliating strain Vd991 and non-defoliating strain D08047) on media separately showed 78.6% and 85% inhibition of V. dahliae, respectively. The product WPBvR001 displayed no phytotoxicity, colonized the plant rhizosphere efficiently, and resulted in significant reduction in the wilt incidence and severity on tobacco and cotton. Optimization of the biocontrol formulation of strain BvR001 was accomplished by mixing diatomaceousearth 87%, sodium dodecyl sulfate (SDS) 5%, sodium lignosulfonate 5%, carboxymethyl cellulose sodium 2%, and vitamin C 1%. The product WPBvR001 contained dehydrated 7.65×108 cfu/g of BvR001 with 99.9% viability and 0 other microorganisms, fineness pass (≤45 μm), wetting time of 38 s, suspension rate of 62.16%, dry decrement of 0.67%, and a pH of 6.89±0.02. Our study demonstrated that the biocontrol strain BvR001 is a valuable new product for the Verticillium wilt management in crops. |
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