| 毕亚琪1, 王禹贺1, 2, 罗泗川1, 郭瑞佳1, 王 晶1, 蒋辅燕1*.MB和CMC液体培养基对禾谷镰孢产孢水平的影响[J].植物保护,2020,46(6):155-158. |
| MB和CMC液体培养基对禾谷镰孢产孢水平的影响 |
| Effects of MB and CMC growth media on the sporulation of Fusarium graminearum |
| 投稿时间：2019-08-01 修订日期：2019-09-30 |
| DOI：DOI:10.16688/j.zwbh.2019393 |
| 中文关键词: 禾谷镰孢 玉米穗腐病 分生孢子 培养基 |
| 英文关键词:Fusarium graminearum maize ear rot conidium culture medium |
| 基金项目:国家自然科学基金(31460351) |
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| 中文摘要: |
| 禾谷镰孢分生孢子定量接种是研究作物抗禾谷镰孢的必要手段?本试验利用绿豆(mung bean, MB)与羧甲基纤维素(carboxylmethyl cellulose, CMC)液体培养基在相同条件下培养禾谷镰孢, 比较两种培养基对禾谷镰孢产孢效率的影响; 并用两种培养基诱导分生孢子制成相同浓度孢子悬浮液, 对75个玉米家系进行人工接种鉴定, 检测不同培养基所产分生孢子致病性差异?结果表明, CMC培养基诱导分生孢子增长率(k2=1.125)大于MB培养基诱导增长率(k1=0.844)?在培养第14天, MB培养基诱导获得的分生孢子平均浓度为2.51×105个/mL, CMC培养基诱导的为3.62×105个/mL, 比MB培养基诱导多44.22%?用两种培养基诱生的孢子进行田间接种, 玉米穗腐病发病程度无明显差异?CMC培养基具有产孢快?孢子浓度高的优点, 是一种适宜禾谷镰孢分生孢子诱生的高效液体培养基? |
| 英文摘要: |
| Artificial inoculation with the conidial suspension of Fusarium graminearum is an important technique for studying the diseases of maize ear and wheat related to F. graminearum. In this study, we used mung bean (MB) medium and carboxylmethyl cellulose (CMC) medium to cultivate the conidia of F. graminearum to compare their cultivating efficiencies. The two kinds of media were used to prepare conidial suspensions for artificial inoculation. The suspensions of F. graminearum from MB and CMC media were separately used to inoculate 75 families of maize recombinant inbred lines (RIL), in order to observe the differences in the infectious potential of the two suspensions. The results showed that, under the same conditions, the growth rates of F. graminearum spores in CMC medium (k2) and MB medium (k1) were 1.125 and 0.844, respectively. Higher growth rate led to higher spore concentration. After 14 days of incubation, the concentrations of F. graminearum conidia in MB medium and CMC medium were 2.51×105 spores/mL and 3.62×105 spores/mL, respectively, and the first was 44.22% higher than the latter. The ear rot identification after artificial inoculation indicated that there was no significant difference in maize ear rot infection between MB and CMC spore suspensions for the same concentration. Therefore, CMC medium was a more effective culture for producing F. graminearum conidia, because more spores could be harvested from CMC medium in a shorter time than from MB medium. |
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