| 王 爽1, 李新民1*, 刘春来1*, 杨 帆1, 刘 亮1, 刘 博2.平贝母鳞茎腐烂病病原菌鉴定和药剂筛选[J].植物保护,2020,46(6):65-70. |
| 平贝母鳞茎腐烂病病原菌鉴定和药剂筛选 |
| Identification of the pathogen of the bulb rot of Fritillaria ussuriensis and screening of the fungicides |
| 投稿时间：2019-04-03 修订日期：2019-06-28 |
| DOI：DOI:10.16688/j.zwbh.2019167 |
| 中文关键词: 平贝母 茎腐病 尖孢镰刀菌 芳香镰刀菌 药剂筛选 |
| 英文关键词:Fritillaria ussuriensis stem rot Fusarium oxysporum F. redolens fungicide selection |
| 基金项目:国家重点研发计划(2017YFD0201108-04); 黑龙江省农业科学院“农业科技创新跨越工程”经济作物科技创新专项(HNK2019CX06-01-03) |
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| 中文摘要: |
| 为明确平贝母Fritillaria ussuriensis鳞茎腐烂病的病原菌及其防治, 对病原菌进行分离?致病性测定, 通过形态学特征?ITS rDNA?β-tubulin及EF-1α基因序列分析确定其病原为尖孢镰刀菌Fusarium oxysporum和芳香镰刀菌F.redolens?通过菌丝生长速率法, 对病原菌进行了室内药剂筛选, 表明8种药剂中25%多菌灵可湿性粉剂和43%戊唑醇悬浮剂对病原菌有更好的抑制作用, 处理第6天, EC50均不超过0.7 mg/L?对两种病原菌防效最好的药剂是戊唑醇, EC50分别为0.107?0.169 mg/L?这两种药剂可以选择应用于平贝母生产来防治茎腐病? |
| 英文摘要: |
| To make clear the fungal pathogen species of the bulb rot of Fritillaria ussuriensis and the control, pathogens were isolated and their pathogenicities were tested. Based on morphological characteristics, ITS rDNA, β-tubulin and EF-1α gene sequences analysis, pathogens were identified were Fusarium oxysporum and F.redolens. Indoor inhibitory activity analysis of eight kinds of fungicides demonstrated that two kinds of fungicides including carbendazim 25% WP and tebuconazole 43% SC had better inhibitory effect on two pathogens. With EC50 values no more than 0.7 mg/L. Results showed that tebuconazole was the best agent to inhibit the mycelia of the two pathogens, with the EC50 values were 0.107 mg/L and 0.169 mg/L, respectively. Thus, these two kinds of fungicides can be recommended as alternative agents for field testing. |
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