| 刘 晨1, 陈志杰1, 杨艺炜1, 郑小慧2, 王家哲1, 张淑莲1, 李英梅1*.陕西省首次发现象耳豆根结线虫危害洛南白菜[J].植物保护,2020,46(5):156-159. |
| 陕西省首次发现象耳豆根结线虫危害洛南白菜 |
| First report of Meloidogyne enterolobii infecting cabbage in Luonan county, Shaanxi province |
| 投稿时间：2019-06-11 |
| DOI：DOI: 10.16688/j.zwbh.2019287 |
| 中文关键词: 陕西省 白菜 种类鉴定 系统发育树 象耳豆根结线虫 |
| 英文关键词:Shaanxi province Chinese cabbage species identification phylogenetic tree Meloidogyne enterolobii |
| 基金项目:陕西省科技厅一般项目（2020NY-072）；西安市农业科技创新工程（20193064YF052NS052）；陕西省科学院一所一品项目（2019K-05）；陕西省科学院科技计划（2019K-12） |
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| 中文摘要: |
| 采用形态学鉴定、特异性引物鉴定及rDNA-ITS序列分析方法, 对采自陕西省洛南县白菜的根结线虫进行了种类鉴定, 并结合构建系统发育树, 确定了感染洛南县白菜的根结线虫为象耳豆根结线虫。切片观察发现，其会阴花纹整体呈圆形至卵圆形, 线纹较密且平滑, 背弓较高, 为方形或近圆形, 无明显侧线。利用象耳豆根结线虫特异性引物进行PCR扩增, 得到大小约236 bp的目的条带, 说明所鉴定线虫为象耳豆根结线虫。系统发育树结果显示, 所鉴定根结线虫与已知的不同地区象耳豆根结线虫聚为同一进化分支, 进一步确定陕西省洛南县采集的根结线虫为象耳豆根结线虫。本文首次发现并报道了陕西省有象耳豆根结线虫为害, 为该线虫的扩散及防治研究奠定了基础。 |
| 英文摘要: |
| The root-knot nematodes collected from Luonan county, Shaanxi province were identified to be Meloidogyne enterolobii by morphological observation, rDNA-ITS sequence analysis, specific primers identification and phylogenetic analysis. Perineal pattern of the root-knot nematode was round to oval, lines were dense and smooth, back bow was square or round and high, and no obvious lateral line. A 236 bp target band could be amplified by specific primers PCR amplification, indicating the root-knot nematode was M. enterolobii. Phylogenetic tree demonstrated that the root-knot nematode was clustered in the same evolutionary branch with those from different regions, thus we further confirmed that the root-knot nematode collected in Luonan county, Shaanxi province was M. enterolobii. |
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