| 许忠顺1, 薛 原2, 张 丽2, 王真娣2, 曾召英1, 杨茂发3, 邹 晓1*.防治斜纹夜蛾蛹和2龄幼虫的棒束孢菌株筛选[J].植物保护,2020,46(5):93-101. |
| 防治斜纹夜蛾蛹和2龄幼虫的棒束孢菌株筛选 |
| Screening of Isaria isolates for controlling the pupae and second-instar larvae of Spodoptera litura |
| 投稿时间：2019-12-10 |
| DOI：DOI: 10.16688/j.zwbh.2019682 |
| 中文关键词: 环链棒束孢 斜纹夜蛾 生物防治 拌土法 LC50 |
| 英文关键词:Isaria cateinannulata Spodoptera litura biological control soil mixing method LC50 |
| 基金项目:黔科合重大专项字［2016］3022-07号；贵州省农业攻关项目［20192405］；中国烟草总公司贵州省公司重大科技专项［201603，201752010040001］；中国烟草总公司贵州省公司科技项目［201712］ |
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| 中文摘要: |
| 为获取对斜纹夜蛾Spodoptera litura (Fabricius)具致病力的生防真菌资源, 通过对8株寄主为鳞翅目蛹的棒束孢属Isaria spp.真菌进行形态学和分子系统学鉴定; 以斜纹夜蛾的蛹和2龄幼虫为试虫, 分别采用拌土法和浸渍法测试菌株的致病力。结果显示:菌株GZUIFR08XS1、GZUIFR04XS8、GZUIFR08XS13为环链棒束孢I. cateinannulata, 菌株GZUIFR08LYS4、GZUIFR04XS5、GZUIFR04XS7、GZUIFR08XS12、GZUIFR04XS16为粉棒束孢I. farinose。8株菌对斜纹夜蛾的蛹和2龄幼虫均有致病力。土壤接种5 mL浓度为2×108 个/mL的分生孢子, 环链棒束孢GZUIFR08XS1、GZUIFR04XS8对蛹的致死率均为100%。接种浓度为1×108 个/mL的分生孢子, 环链棒束孢GZUIFR04XS8对2龄幼虫致死率为(78.21±2.22)%, 较其他菌株有差异显著(P<0.05)。将环链棒束孢GZUIFR04XS8分别接种在2~5龄幼虫时, 对同一龄期幼虫的致死率随接种浓度的增大而上升, 其中, 接种孢子浓度为1×109 个/mL可致2龄幼虫100%死亡; 同一接种浓度下, 对幼虫致死率随幼虫龄期的增大而下降, 其中, 2龄幼虫的致死中浓度LC50为1.28×105 个/mL。环链棒束孢GZUIFR04XS8有较好防治斜纹夜蛾蛹和低龄幼虫的生防潜力。 |
| 英文摘要: |
| In order to obtain biocontrol fungus resources with high pathogenicity to Spodoptera litura (Fabricius), eight isolates of Isaria spp. with the pupae of Lepidoptera as their hosts were identified by morphology and molecular systematicS. Using pupae and 2nd-instar larvae of S. litura as test insects, the pathogenicity of isolates was tested by using soil mixing and dipping methods, respectively. The results showed that the isolates GZUIFR08XS1, GZUIFR04XS8 and GZUIFR08XS13 were I. cateinannulata, and the isolates GZUIFR08LYS4, GZUIFR04XS5, GZUIFR04XS7, GZUIFR08XS12 and GZUIFR04XS16 were I. farinose. The eight isolates were pathogenic to the 2nd-instar larvae and pupae of S. litura. When the soil was inoculated with 5 mL of 2×108 spores/mL, and the corrected mortality rate of the pupae of S. litura caused by GZUIFR08XS1 and GZUIFR04XS8 was 100%. When 1×108 spores/mL were inoculated, the mortality rate of the 2nd-instar larvae caused by GZUIFR04XS8 was (78.21 ± 2.22)%, which was significantly different from other strains (P<0.05). When the 2nd-5th instar larvae were inoculated with GZUIFR04XS8, the mortality of larvae at the same age increased with increasing inoculation concentration. Inoculation of 1×109 spores/mL caused 100% death of 2nd instar larvae. At the same inoculation concentration, the larval mortality decreased with the increase of instar. The LC50 value of the 2nd-instar larvae was 1.28×105 spores/mL. I. cateinannulata GZUIFR04XS8 showed a good biocontrol potential for controlling pupae and young larvae of S. litura. |
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