| 许铜硕, 刘太国, 刘 博, 陈万权, 高 利*.转录组测序及其在植物病原菌、植物与真菌互作研究中的应用与进展[J].植物保护,2020,46(5):13-17. |
| 转录组测序及其在植物病原菌、植物与真菌互作研究中的应用与进展 |
| Application and progresses in transcriptome sequencing for the study of plant pathogens and plant-fungal interactions |
| 投稿时间：2019-07-15 |
| DOI：DOI: 10.16688/j.zwbh.2019358 |
| 中文关键词: 转录组测序 寄主 病原物 |
| 英文关键词:RNA-seq host pathogen |
| 基金项目:国家重点研发计划(2018YFD0200406); 国家自然科学基金国际(组织间)合作项目(31761143011)；国家自然科学基金(31571965) |
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| 中文摘要: |
| 转录组测序(RNA sequencing, RNA-seq)技术是用来分析动态转录组的重要工具。RNA-seq生成的数据不仅可以量化基因表达, 还有助于发现新的转录本、识别选择性剪接基因和检测等位基因特异性表达。本文主要综述了RNA-seq的技术步骤及工作原理, 并介绍了其在植物病原菌及植物与真菌互作研究中的应用与进展。 |
| 英文摘要: |
| Transcriptome sequencing (RNA-seq) is an important tool for analyzing dynamic transcriptomes. In addition to quantifying gene expression, RNA-seq generated data helping discover new transcripts, recognize alternative splicing genes and detect allele-specific expression. This paper mainly reviewed the technical steps and working principles of RNA-seq and introduced its application and progresses in the study of plant pathogens and plant-fungal interactions. |
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