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邹德勇1,2, 杨智越3, 杜春梅1,2*.Phaeosphaeria sp.HD-06菌株对马唐的致病性分析及初步鉴定[J].植物保护,2020,46(4):98-104.
Phaeosphaeria sp.HD-06菌株对马唐的致病性分析及初步鉴定
Pathogenicity of Phaeosphaeria sp. HD-06 against Digitaria sanguinalis and the preliminary identification of the strain
投稿时间:2019-04-03  修订日期:2019-06-03
DOI:10.16688/j.zwbh.2019166
中文关键词:  马唐  生防菌  致病性  鉴定
英文关键词:Digitaria sanguinalis  biocontrol bacteria  pathogenicity  identification
基金项目:国家自然科学基金(31370511);国家重点研发计划(2017YFD0201101)
作者单位E-mail
邹德勇1,2, 杨智越3, 杜春梅1,2* 1. 黑龙江大学农业微生物技术教育部工程研究中心, 哈尔滨 150500
2. 黑龙江大学生命科学学院, 黑龙江省普通高校微生物重点实验室, 哈尔滨 150080
3. 哈尔滨兴普饲料有限公司, 哈尔滨 150010 
1487598102@qq.com 
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中文摘要:
      为了得到能够有效抑制马唐Digitaria sanguinalis生长的生防微生物,在农田采集马唐种子,进行萌发试验,观察种子的萌发状况,从发病的种子和马唐幼苗基部分离致病菌,通过形态学观察以及ITS 序列分析对致病菌进行鉴定。结果从罹病的马唐幼苗基部分离得到一株致病菌HD-06,该菌株的发酵液用水稀释至60%浓度时,对马唐种子的萌发抑制率和幼苗发病率均达到100%,这说明菌株HD-06对马唐有较好的生物防治作用。根据其形态学特点及ITS 序列分析结果,鉴定菌株HD-06为暗球腔菌属Phaeosphaeria的真菌。这些结果为深入研究其对马唐的防治作用奠定了基础。
英文摘要:
      In order to obtain the effective biocontrol microorganism for inhibiting the growth of Digitaria sanguinalis, the seeds of crabgrass were collected in the farmland, and seed germination test was carried out. The germinating situation was tracked over time; meanwhile, the pathogens were isolated from the diseased seeds and the seedling base, and morphological observation and ITS sequence analysis were conducted to identify the pathogenic fungi. The results showed that a disease-causing strain named HD-06 was successfully isolated from diseased crabgrass seedling base. When the fermentation broth of strain HD-06 was diluted by 60% with water, the germination inhibition rate and seedling disease incidence rate of crabgrass reached 100% after treatment. It indicated that strain HD-06 had a good biological control effect on crabgrass. According to the morphological characteristics and ITS sequence analysis results, strain HD-06 was identified as a fungus of the genus Phaeosphaeria. These results laid a foundation for further research on its control effect on crabgrass.
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