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张燕娜, 赵 倩, 赵伊英*.Wolbachia对土耳其斯坦叶螨生殖影响的转录组测序分析[J].植物保护,2020,46(4):81-90.
Wolbachia对土耳其斯坦叶螨生殖影响的转录组测序分析
Transcriptome sequencing and analysis of the effects of Wolbachia on thereproduction of Tetranychus turkestani
投稿时间:2018-09-06  修订日期:2018-11-19
DOI:10.16688/j.zwbh.2018389
中文关键词:  土耳其斯坦叶螨  Wolbachia  转录组  生殖调控
英文关键词:Tetranychus turkestani  Wolbachia  transcriptome  reproductive regulation
基金项目:国家自然科学基金(31860508)
作者单位E-mail
张燕娜, 赵 倩, 赵伊英* 石河子大学农学院, 石河子 832000 2235804670@qq.com 
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中文摘要:
      为进一步探索内共生菌Wolbachia对土耳其斯坦叶螨生殖调控的影响,本试验筛选出完全感染Wolbachia和完全不感染的土耳其斯坦叶螨品系进行转录组测序。结果表明:感染Wolbachia后,叶螨体内一些与生殖相关的基因发生明显变化,其中3 810个基因在雌成螨中受到影响,2 885个基因在雄成螨中受到影响;其中一些参与脂质转运、氧化还原反应、消化及解毒作用的基因具有明显的性别特异性。这为内共生菌Wolbachia引起宿主生殖调控提供了新的理论依据。
英文摘要:
      To further explore the effects of endosymbiotic bacteria Wolbachia on reproduction of Tetranychus turkestani, the completely single-infected and uninfected T.turkestani lines were established and their transcriptomes were sequenced. The results showed that after infection with Wolbachia, some genes related to reproduction in the mites showed significant change, in which 3 810 genes were affected in female adult mites and 2 885 genes were affected in male mites. Some of the genes involved in lipid transport, redox reaction, digestion and detoxification were closely related to gender specific. Our study provides a new theoretical basis for the host reproductive regulation by Wolbachia.
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